Human Phospho-STAT3 (Y705) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB46071-100
MAB46071-SP
Detection of Human Phospho-STAT3 (Y705) by Western Blot.
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Product Details
Citations (2)
FAQs
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Human Phospho-STAT3 (Y705) Antibody Summary

Species Reactivity
Human
Specificity
Detects human STAT3 when phosphorylated at Y705 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG2B Clone # 1004G
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phosphopeptide containing the human STAT3 Y705 site
Accession # P40763
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

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Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-STAT3 (Y705) antibody by Western Blot. View Larger

Detection of Human Phospho-STAT3 (Y705) by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-22 (Catalog # 782-IL) for 15 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-STAT3 (Y705) Monoclonal Antibody (Catalog # MAB46071) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT3 (Y705) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry Detection of Phospho-STAT3 (Y705) antibody in IFN alpha-treated Daudi Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Phospho-STAT3 (Y705) in IFN alpha-treated Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was unstimulated (open histogram) or treated with 500 U/mL rhIFN-alpha for 20 minutes (filled histogram) and stained with Rabbit anti-Human Phospho-STAT3 (Y705) Molyclonal Antibody (Catalog # MAB46071) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT3

Human STAT-3/STAT3a (signal transducer and activator of transcription 3; also called APRF) is a 90-95 kDa member of the STAT family of transcription factors. It is 770 amino acids (aa) in length, contains one SH2 domain (aa 580-670), and is found in almost all cell types. STAT3 mediates gp130, LIF-R, OB-R, IL10-R and EGFR signaling. Upon activation, receptors such as gp130 phosphorylate STAT3 at Ser727, which results in transcriptional activation. Phosphorylation at Tyr705 mediates homo- and hetero-dimerization with STAT1 and nuclear translocation, and is an important event in oncogenic transformation. The STAT3b isoform shows a six aa substitution for aa 716-770, thus eliminating the Ser727 site. Human and mouse STAT3a share 99% aa sequence identity.

Long Name
Signal Transducer and Activator of Transcription 3
Entrez Gene IDs
6774 (Human); 20848 (Mouse); 25125 (Rat)
Alternate Names
Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3; STAT3

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Citations for Human Phospho-STAT3 (Y705) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Human omental adipose-derived mesenchymal stem cells enhance autophagy in ovarian carcinoma cells through the STAT3 signalling pathway
    Authors: Y Chu, Y Wang, K Li, M Liu, Y Zhang, Y Li, X Hu, C Liu, H Zhou, J Zuo, W Peng
    Cell. Signal., 2020;69(0):109549.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Adipose-derived mesenchymal stem cells promote osteosarcoma proliferation and metastasis by activating the STAT3 pathway
    Authors: Y Wang, Y Chu, B Yue, X Ma, G Zhang, H Xiang, Y Liu, T Wang, X Wu, B Chen
    Oncotarget, 2017;8(14):23803-23816.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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