Human Phospho-STAT6 Y641 Antibody

Catalog # Availability Size / Price Qty
MAB37172
MAB37172-SP
Phospho-STAT6  Y641 in U937 Human Cell Line.
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Human Phospho-STAT6 Y641 Antibody Summary

Species Reactivity
Human
Specificity
Detects human STAT6 when phosphorylated at Y641.
Source
Monoclonal Rat IgG2B Clone # 943015
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing the human STAT6 Y641 site
Accession # P42226
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Phospho-STAT6  Y641 in U937 Human Cell Line. View Larger

Phospho-STAT6 Y641 in U937 Human Cell Line. STAT6phosphorylated at Y641 was detected in immersion fixed U937 human histiocytic lymphoma cell line treated with Recombinant Human IL-4 (Catalog # 204-IL) using Rat Anti-Human Phospho-STAT6 Y641 Monoclonal Antibody (Catalog # MAB37172) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT6

Signal Transducer and Activator of Transcription 6 (STAT6) mediates the signaling of cytokines such as IL-4 and IL-13. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN4GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins.

Long Name
Signal Transducer and Activator of Transcription 6
Entrez Gene IDs
6778 (Human); 20852 (Mouse); 362896 (Rat)
Alternate Names
D12S1644; EC 2.4.1.227; EC 2.7.7.6; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin4-induced; STAT6; STAT6B; STAT6C; transcription factor IL-4 STAT

Product Datasheets

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Cell and Tissue Staining Kits

Isotype Controls

Rat IgG2B Isotype Control

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Reconstitution Buffers

Secondary Antibodies

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