Human pIgR Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human pIgR in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse pIgR is observed.
  • Source
    Monoclonal Mouse IgG3 Clone # 825724
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human pIgR
    Lys19-Arg638
    Accession # CAA51532
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Simple Western
    2.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human pIgR by Western Blot. Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human pIgR Monoclonal Antibody (Catalog # MAB2717) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for pIgR at approximately 85-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human pIgR by Simple WesternTM. Simple Western lane view shows lysates of human stomach tissue, loaded at 1 mg/mL. A specific band was detected for pIgR at approximately 110-150 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human pIgR Monoclonal Antibody (Catalog # MAB2717). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: pIgR

The human polymeric immunoglobulin receptor (pIgR; also known as membrane secretory component) is an approximately 100 kDa type I transmembrane glycoprotein that is synthesized as a 764 amino acid (aa) precursor. It includes a signal sequence (aa 1-18), an extracellular region (aa 19-638), a transmembrane segment (aa 639-661), and a cytoplasmic domain (aa 662-764) (1-3). The extracellular region consists of five Ig-like domains and a sixth non-Ig domain that connects to the membrane region. pIgR is expressed on secretory epithelial cells of exocrine tissues. Immunoglobulin isotypes consist of two heavy (H) and two light (L) chains. For IgA and IgM, this H2L2 monomer can form larger polymers through association with a joining chain (J chain). The Fc regions of IgA and IgM have a carboxy-terminal extension called a secretory tailpiece that binds the J chain (4). pIgR functions as a carrier that transports IgA and IgM across epithelium (5). On the basolateral surface of epithelial cells, the receptor initially binds non-covalently to IgA via a docking site on the J chain. This initiates a rearrangement in which a disulfide bond forms between pIgR and an IgA heavy chain (2). The complexes are then internalized and transcytosed to the apical surface. A soluble covalent complex called secretory IgA (SIgA) is now generated by proteolytic cleavage of the sixth extracellular domain of pIgR and released into the lumen (6). This IgA-bound and proteolytically generated pIgR fragment is referred to as secretory component (SC). Notably, human pIgR transcytoses constitutively, with or without ligand, creating both bound and free, 78 kDa SC following cleavage (3). The extracellular region of pIgR shares 64%, 65%, and 70% aa sequence identity with the equivalent region of rat, mouse and porcine pIgR, respectively. The receptor component of the complex anchors the SIgA molecule to mucous (7). SIgA is a crucial component of the mucosal immune system serving to protect the large expanse of mucous membranes that form a barrier between the interior of the body and the external environment (8).

  • References:
    1. Krajci, P. et al. (1989) Biochem. Biophys. Res. Commun. 158:783.
    2. Piskurich, J. et al. (1995) J. Immunol. 154:1735.
    3. Brandtzaeg, P. and F-E. Johansen (2001) Trends Immunol. 22:545.
    4. Braathen, R. et al. (2002) J. Biol. Chem. 277:42755.
    5. Ben-Hur, H. et al. (2004) Int. J. Mol. Med. 14:35.
    6. Asano, M. et al. (2004) Immunology 112:583.
    7. Phalipon, A. and B. Corthesy (2003) Trends Immunol. 24:55.
    8. Uren, T. et al. (2003) J. Immunol. 170:2531.
  • Long Name:
    Polymeric Immunoglobulin Receptor
  • Entrez Gene IDs:
    5284 (Human); 18703 (Mouse)
  • Alternate Names:
    FLJ22667; hepatocellular carcinoma associated protein TB6; Hepatocellular carcinoma-associated protein TB6; MGC125361; MGC125362; pIgR; Poly-Ig receptor; polymeric immunoglobulin receptor
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Streptococcus pneumoniae Interacts with pIgR expressed by the brain microvascular endothelium but does not co-localize with PAF receptor.
    Authors: Iovino, Federico, Molema, Grietje, Bijlsma, Jetta J
    PLoS ONE, 2014;9(5):e97914.
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG3 Isotype Control

Ctrl MAB007 3  
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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