Plasminogen (PLG) is the precursor of plasmin, an active serine protease that dissolves the fibrin of blood clots and acts in many other processes such as embryonic development, tissue remodeling, inflammation, and tumor invasion (1, 2). Synthesized in the kidney, PLG is found in plasma and many extracellular fluids. Activated by u- or t-plasminogen activator, the single-chain PLG (amino acid residues 20-810) is converted to plasmin, which consists of disulfide bond-linked heavy chain A (residues 20-580) and light chain B (residues 581-810). Heavy chain A contains 5 kringle domains and light chain B corresponds to the serine protease domain. A fragment consisting of the first 4 kringle domains has been named as angiostatin, a novel angiogenesis inhibitor (3, 4).
Human Plasminogen Antibody
R&D Systems | Catalog # MAB1939
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Glu20-Asn810
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Plasminogen Antibody
Neutralization of Plasminogen Activity by Human Plasminogen Antibody.
The cleavage of Suc-AFK-AMC (100 µM) by Human Plasminogen (1 µg/mL, Catalog # 1939-SE) is measured after preincubation with increasing concentrations of Mouse Anti-Human Plasminogen Monoclonal Antibody (Catalog # MAB1939). The ND50 is typically 1 µg/mL.
Detection of Plasminogen by Western Blot
Diminished PLG in psoriasis.(A) A significant reduction in the expression of PLG in PBMC of psoriasis patients was detected by flow cytometry. (B) Cryosections from lesional skin of psoriasis patients or normal volunteers were stained with a PLG mAb (red). (C) Lysates from lesional skin of psoriasis patients or normal skin of volunteers were subjected to western blotting for PLG expression. Human PLG was used as a positive control (right panel). Actin - loading control. (D, E) Cryosections from lesional skin of psoriasis patients or normal volunteers were stained with uPA or tPA mAbs (green) for its expression. Original magnification for immunofluorescent staining, ×400. e, epidermis; d, dermis. Dotted lines indicate the border between epidermis and dermis. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/21311769), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Plasminogen Antibody
Immunoprecipitation
Sample: Conditioned cell culture medium spiked with Human Plasminogen (Catalog # 1939-SE), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Human Plasminogen (1 µg/mL, Catalog # 1939-SE) cleavage of the fluorogenic peptide substrate Suc-AFK-Amc (100 µM). The Neutralization Dose (ND50) is typically 1 µg/mL.
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Plasminogen
References
- Petersen, T.E. et al. (1990) J. Biol. Chem. 265:6104.
- Forsgren, M. et al. (1987) FEBS Lett. 213:254.
- O’Reilly, M.S. et al. (1994) Cell 79:315.
- Sim, B.K. et al. (1997) Cancer Res. 57:1329.
Alternate Names
Gene Symbol
Additional Plasminogen Products
Product Documents for Human Plasminogen Antibody
Product Specific Notices for Human Plasminogen Antibody
For research use only
Citations for Human Plasminogen Antibody
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