Measured by its ability to neutralize Human Plasminogen (1 µg/mL, Catalog # 1939-SE) cleavage of the fluorogenic peptide substrate Suc-AFK-Amc (100 µM). The Neutralization Dose (ND50) is typically 1 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Neutralization of Plasminogen Activity by Human Plasminogen Antibody.
The cleavage of Suc-AFK-AMC (100 µM) by Human Plasminogen (1 µg/mL, Catalog # 1939-SE) is measured after preincubation with increasing concentrations of Mouse Anti-Human Plasminogen Monoclonal Antibody (Catalog # MAB1939). The ND50 is typically 1 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Plasminogen (PLG) is the precursor of plasmin, an active serine protease that dissolves the fibrin of blood clots and acts in many other processes such as embryonic development, tissue remodeling, inflammation, and tumor invasion (1, 2). Synthesized in the kidney, PLG is found in plasma and many extracellular fluids. Activated by u- or t-plasminogen activator, the single-chain PLG (amino acid residues 20-810) is converted to plasmin, which consists of disulfide bond-linked heavy chain A (residues 20-580) and light chain B (residues 581-810). Heavy chain A contains 5 kringle domains and light chain B corresponds to the serine protease domain. A fragment consisting of the first 4 kringle domains has been named as angiostatin, a novel angiogenesis inhibitor (3, 4).
Petersen, T.E. et al. (1990) J. Biol. Chem. 265:6104.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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