Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human

Applications

Validated:

Immunohistochemistry, ELISA Detection (Matched Antibody Pair)

Cited:

Immunohistochemistry-Paraffin, ELISA Capture, ELISA Detection, ELISA Development, ELISA Development (Detection), Functional Assay, Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all IL-17/IL-17A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552

Specificity

Detects human and primate IL-17 in direct ELISAs. In sandwich immunoassays, approximately 22% cross-reactivity with rhIL-17A/F heterodimer is observed, and less than 0.05% cross‑reactivity with recombinant mouse (rm) IL‑17, recombinant human (rh) IL-10, rhIL-12, rhIL-16, rhIFN-gamma, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E and rhIL-17F is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Primate IL‑17/IL‑17A Biotinylated Antibody

IL-17/IL-17A antibody in Human Crohn's Disease Intestine by Immunohistochemistry (IHC-P).

IL‑17/IL‑17A in Human Crohn's Disease Intestine.

IL-17/IL-17A was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Goat Anti-Human/Primate IL-17/IL-17A Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF317) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Primate IL‑17/IL‑17A Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

0.3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Crohn's disease intestine

Human/Primate IL-17 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human/Primate IL‑17/IL‑17A Antibody (Catalog # MAB317)
  • Standard: Recombinant Human IL-17A Protein, CF (Catalog # 317-ILB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using BAF317 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17/IL-17A

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide-linked homodimers. At the amino acid level, human IL-17 shows 72% and 63% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation. The non-glycosylated, E. coli-expressed rhIL-17 has been shown to be active on human as well as mouse cell lines.

References

  1. Yao, Z. et al. (1995) J. Immunol. 155:5483.
  2. Yao, Z. et al. (1995) Immunity 3:811.
  3. Rouvier, E. et al. (1993) J. Immunol. 150:5445.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Gene Symbol

IL17A

UniProt

Q16552

Additional IL-17/IL-17A Products

Product Documents for Human/Primate IL‑17/IL‑17A Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Primate IL‑17/IL‑17A Biotinylated Antibody

For research use only

Citations for Human/Primate IL‑17/IL‑17A Biotinylated Antibody

Customer Reviews for Human/Primate IL‑17/IL‑17A Biotinylated Antibody (2)

4.5 out of 5
2 Customer Ratings
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  • Human/Primate IL-17/IL-17A Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Cell culture supernatant
    Species: Human
    Verified Customer | Posted 08/22/2018
    The antibody was used as a secondary in an IL17A immunoassay.
    Human/Primate IL‑17/IL‑17A Biotinylated Antibody BAF317
  • Human/Primate IL-17/IL-17A Biotinylated Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 01/18/2018

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Protocols

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