Human Prolactin Antibody

Catalog # Availability Size / Price Qty
MAB6821
MAB6821-SP
Human Prolactin Antibody in Western Blot
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Product Details
Citations (1)
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Human Prolactin Antibody Summary

Species Reactivity
Human
Specificity
Recognizes both the full-length (amino acids 29 - 229) and a truncated form (amino acids 29 - 151) of recombinant human Prolactin in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 127813
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Prolactin
Leu29-Thr151
Accession # P01236
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Prolactin by Western Blot. View Larger

Detection of Human Prolactin by Western Blot. Western blot shows lysate of human pituitary tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Prolactin Monoclonal Antibody (Catalog # MAB6821) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prolactin

Prolactin (PRL) is a neuroendocrine pituitary hormone that is synthesized by anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, natural killer cells, and breast cancer cells. The prolactin receptor is a transmembrane type I glycoprotein that belongs to the hematopoietic cytokine receptor family.

Prolactin receptor (PRL R) contains an extracellular, transmembrane, and intracellular domain. Transcriptional regulation of the PRL R gene results in several different species-dependent isoforms of PRL R being produced.

Entrez Gene IDs
5617 (Human); 19109 (Mouse)
Alternate Names
PRL; Prolactin

Product Datasheets

Citation for Human Prolactin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Prolactin receptor antagonism reduces the clonogenic capacity of breast cancer cells and potentiates doxorubicin and paclitaxel cytotoxicity.
    Authors: Howell SJ, Anderson E, Hunter T
    Breast Cancer Res., 2008;10(4):R68.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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