Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 127813
View all Prolactin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Prolactin
Leu29-Thr151
Accession # P01236

Specificity

Recognizes both the full-length (amino acids 29 - 229) and a truncated form (amino acids 29 - 151) of recombinant human Prolactin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Prolactin Antibody (127813)

Detection of Human Prolactin antibody by Western Blot.

Detection of Human Prolactin by Western Blot.

Western blot shows lysate of human pituitary tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Prolactin Monoclonal Antibody (Catalog # MAB6821) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Prolactin Antibody (127813)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human pituitary tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Prolactin

Prolactin (PRL) is a secreted neuroendocrine hormone that acts primarily on the mammary gland to promote lactation. It is secreted by the anterior pituitary under tonic inhibition by hypothalamic dopamine. In humans Prolactin is also produced peripherally, while in rodents, the only known non-pituitary source is the placenta. Prolactin bioactivity is regulated by post-translational modifications including polymerization, phosphorylation, and proteolytic cleavage. Cleavage by matrix metalloproteinases or Cathepsin D can produce antiangiogenic fragments also called vasoinhibins.

Alternate Names

PRL

Entrez Gene IDs

5617 (Human); 19109 (Mouse)

Gene Symbol

PRL

UniProt

P01236

Additional Prolactin Products

Product Documents for Human Prolactin Antibody (127813)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Prolactin Antibody (127813)

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human Prolactin Antibody (127813)

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