Human Prolactin Antibody Summary
Accession # P01236
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Prolactin by Western Blot. Western blot shows lysate of human pituitary tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Prolactin Monoclonal Antibody (Catalog # MAB6821) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Prolactin (PRL) is a neuroendocrine pituitary hormone that is synthesized by anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, natural killer cells, and breast cancer cells. The prolactin receptor is a transmembrane type I glycoprotein that belongs to the hematopoietic cytokine receptor family.
Prolactin receptor (PRL R) contains an extracellular, transmembrane, and intracellular domain. Transcriptional regulation of the PRL R gene results in several different species-dependent isoforms of PRL R being produced.
Citation for Human Prolactin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Prolactin receptor antagonism reduces the clonogenic capacity of breast cancer cells and potentiates doxorubicin and paclitaxel cytotoxicity.
Authors: Howell SJ, Anderson E, Hunter T
Breast Cancer Res., 2008;10(4):R68.
Sample Types: Cell Culture Supernates
Applications: Western Blot
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