Human Proprotein Convertase 9/PCSK9 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB38883-100
MAB38883-SP
Human Proprotein Convertase 9/PCSK9 ELISA Standard Curve.
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Human Proprotein Convertase 9/PCSK9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Proprotein Convertase 9/PCSK9 in direct ELISAs.
Source
Recombinant Monoclonal Rat IgG2B Clone # 499920R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Proprotein Convertase 9/PCSK9
Arg29-Gln692
Accession # Q8NBP7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Human Proprotein Convertase 9/PCSK9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3888).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Proprotein Convertase 9/PCSK9 DuoSet ELISA Kit (Catalog # DY3888) for convenient development of a sandwich ELISA or the Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit (Catalog # DPC900) for a complete optimized ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human Proprotein Convertase 9/PCSK9 ELISA Standard Curve. Recombinant Human Proprotein Convertase 9/PCSK9 protein was serially diluted 2-fold and captured by Rat Anti-Human Proprotein Convertase 9/PCSK9 Monoclonal Antibody (Catalog # MAB38883) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Proprotein Convertase 9/PCSK9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3888) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Proprotein Convertase 9/PCSK9

The human PCSK9 gene encodes Proprotein Convertase 9 (PC9), which is also known as Neural Apoptosis Regulated Convertase 1 (NARC1) (1). The deduced amino acid sequence of human PCSK9 consists of a signal peptide (aa 1 to 30), a propeptide (aa 31 to 152), and a mature chain (aa 153 to 692) that contains a serine protease domain (aa 161 to 431) found in members of the furin/PC family. PCSK9 protease activity may be limited, since it has only been demonstrated through its own autocatalytic processing (2). After the autocleavage in the ER, the pro domain and mature chain exit the cell together through non‑covalent interactions (3). PCSK9 is a key regulator of LDL-cholesterol levels (LDL-C) through binding of the LDL receptor, resulting in the reduction of receptor recycling to the cell surface and the acceleration of receptor degradation in lysosomes (3). Both gain of function (GOF) and loss-of-function (LOF) mutations have been found in the PCSK9 gene (3). GOF mutations are linked to familial autosomal dominant hypercholesterolemia, a disease characterized by elevated plasma levels of LDL-C. In comparison, LOF mutations lead to low levels of LDL-C and protection against coronary heart disease.

References
  1. Seidah, N.G. et al. (2003) Proc. Natl. Acad. Sci. USA 100:928.
  2. Naureckiene, S. et al. (2003) Arch. Biochem. Biophys. 420:55.
  3. Costet, P. et al. (2008) Trends Biochem. Sci. 33:426.
Entrez Gene IDs
255738 (Human); 100102 (Mouse); 298296 (Rat); 102142788 (Cynomolgus Monkey)
Alternate Names
EC 3.4.21; EC 3.4.21.111; FH3; FH3neural apoptosis regulated convertase 1; FHCL3; HCHOLA3; hypercholesterolemia, autosomal dominant 3; LDLCQ1; NARC1; NARC-1; NARC-1convertase subtilisin/kexin type 9 preproprotein; NARC1EC 3.4.21.-; Neural apoptosis-regulated convertase 1; PC9; PCSK9; Proprotein Convertase 9; proprotein convertase subtilisin/kexin type 9; Subtilisin/kexin-like protease PC9

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