Human Proprotein Convertase 9/PCSK9 Antibody

Catalog # Availability Size / Price Qty
AF3888
AF3888-SP
Detection of Human Proprotein Convertase 9/PCSK9 by Western Blot.
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Product Details
Citations (5)
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Human Proprotein Convertase 9/PCSK9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PCSK9 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse PCSK9 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PCSK9 isoform 1
Gln31-Gln692
Accession # Q8NBP7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Human Proprotein Convertase 9/PCSK9 Monoclonal Antibody (Catalog # MAB38883).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Proprotein Convertase 9/PCSK9 DuoSet ELISA Kit (Catalog # DY3888) for convenient development of a sandwich ELISA or the Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit (Catalog # DPC900) for a complete optimized ELISA.

 
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Proprotein Convertase 9/PCSK9 (Catalog # 3888-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Proprotein Convertase 9/PCSK9 by Western Blot. View Larger

Detection of Human Proprotein Convertase 9/PCSK9 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Proprotein Convertase 9/PCSK9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3888) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pre-Proprotein Convertase 9/PCSK9 at approximately 80 kDa and mature Proprotein Convertase 9/PCSK9 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human Proprotein Convertase 9/PCSK9 ELISA Standard Curve. Recombinant Human Proprotein Convertase 9/PCSK9 protein was serially diluted 2-fold and captured by Rat Anti-Human Proprotein Convertase 9/PCSK9 Monoclonal Antibody (Catalog # MAB38883) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Proprotein Convertase 9/PCSK9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3888) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Proprotein Convertase 9/PCSK9

The human PCSK9 gene encodes Proprotein Convertase 9 (PC9), which is also known as Neural Apoptosis Regulated Convertase 1 (NARC1) (1). The deduced amino acid sequence of human PCSK9 consists of a signal peptide (residues 1-30), a pro peptide (residue 31-152), and a mature chain (residues 153-692) that contains a serine protease domain (residues 161-431) found in members of the furin/PC family. PCSK9 protease activity may be limited, since it has only been demonstrated through its own autocatalytic processing (2). After the autocleavage in the ER, the pro domain and mature chain exit the cell together through non-covalent interactions (3). PCSK9 is a key regulator of LDL-cholesterol levels (LDL-C) through binding of the LDL receptor, resulting in the reduction of receptor recycling to the cell surface and the acceleration of receptor degradation in lysosomes (3). Both gain of function (GOF) and loss-of-function (LOF) mutations have been found in the PCSK9 gene (3). GOF mutations are linked to familial autosomal dominant hypercholesterolemia, a disease characterized by elevated plasma levels of LDL-C. In comparison, LOF mutations lead to low levels of LDL-C and protection against coronary heart disease.

References
  1. Seidah, N.G. et al. (2003) Proc. Natl. Acad. Sci. USA 100:928.
  2. Naureckiene, S. et al. (2003) Arch. Biochem. Biophys. 420:55.
  3. Costet, P. et al. (2008) Trends Biochem. Sci. 33:426.
Entrez Gene IDs
255738 (Human); 100102 (Mouse); 298296 (Rat)
Alternate Names
EC 3.4.21; EC 3.4.21.111; FH3; FH3neural apoptosis regulated convertase 1; HCHOLA3; hypercholesterolemia, autosomal dominant 3; LDLCQ1; NARC-1; NARC-1convertase subtilisin/kexin type 9 preproprotein; NARC1EC 3.4.21.-; Neural apoptosis-regulated convertase 1; PC9; PCSK9; Proprotein Convertase 9; proprotein convertase subtilisin/kexin type 9; Subtilisin/kexin-like protease PC9

Product Datasheets

Citations for Human Proprotein Convertase 9/PCSK9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. 6-Gingerol Regulates Hepatic Cholesterol Metabolism by Up-regulation of LDLR and Cholesterol Efflux-Related Genes in HepG2 Cells
    Authors: X Li, J Guo, N Liang, X Jiang, Y Song, S Ou, Y Hu, R Jiao, W Bai
    Front Pharmacol, 2018;9(0):159.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  2. Heparan sulfate proteoglycans present PCSK9 to the LDL receptor
    Authors: C Gustafsen, D Olsen, J Vilstrup, S Lund, A Reinhardt, N Wellner, T Larsen, CBF Andersen, K Weyer, JP Li, PH Seeberger, S Thirup, P Madsen, S Glerup
    Nat Commun, 2017;8(1):503.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Common Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Epitopes Mediate Multiple Routes for Internalization and Function.
    Authors: DeVay R, Yamamoto L, Shelton D, Liang H
    PLoS ONE, 2015;10(4):e0125127.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  4. Loss of plasma proprotein convertase subtilisin/kexin 9 (PCSK9) after lipoprotein apheresis.
    Authors: Tavori H, Giunzioni I, Linton M, Fazio S
    Circ Res, 2013;113(12):1290-5.
    Species: Human
    Sample Types: Plasma
    Applications: WB
  5. Antibody-mediated disruption of the interaction between PCSK9 and the low-density lipoprotein receptor.
    Authors: Duff CJ, Scott MJ, Kirby IT, Hutchinson SE, Martin SL, Hooper NM
    Biochem. J., 2009;419(3):577-84.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Neut

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