Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunoprecipitation

Cited:

Western Blot, Neutralization, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Proprotein Convertase 9/PCSK9 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PCSK9 isoform 1
Gln31-Gln692
Accession # Q8NBP7

Specificity

Detects human PCSK9 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse PCSK9 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Proprotein Convertase 9/PCSK9 Antibody

Detection of Human Proprotein Convertase 9/PCSK9 antibody by Western Blot.

Detection of Human Proprotein Convertase 9/PCSK9 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Proprotein Convertase 9/PCSK9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3888) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pre-Proprotein Convertase 9/PCSK9 at approximately 80 kDa and mature Proprotein Convertase 9/PCSK9 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Human Proprotein Convertase 9/PCSK9 Antibody in ELISA Standard Curve.

Human Proprotein Convertase 9/PCSK9 ELISA Standard Curve.

Recombinant Human Proprotein Convertase 9/PCSK9 protein was serially diluted 2-fold and captured by Rat Anti-Human Proprotein Convertase 9/PCSK9 Monoclonal Antibody (Catalog # MAB38883) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Proprotein Convertase 9/PCSK9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3888) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human Proprotein Convertase 9/PCSK9 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Human Proprotein Convertase 9/PCSK9 Monoclonal Antibody (Catalog # MAB38883).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Proprotein Convertase 9/PCSK9 DuoSet ELISA Kit (Catalog # DY3888) for convenient development of a sandwich ELISA or the Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit (Catalog # DPC900) for a complete optimized ELISA.

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Proprotein Convertase 9/PCSK9 (Catalog # 3888-SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line

Reviewed Applications

Read 6 reviews rated 4 using AF3888 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Proprotein Convertase 9/PCSK9

The human PCSK9 gene encodes Proprotein Convertase 9 (PC9), which is also known as Neural Apoptosis Regulated Convertase 1 (NARC1) (1). The deduced amino acid sequence of human PCSK9 consists of a signal peptide (residues 1-30), a pro peptide (residue 31-152), and a mature chain (residues 153-692) that contains a serine protease domain (residues 161-431) found in members of the furin/PC family. PCSK9 protease activity may be limited, since it has only been demonstrated through its own autocatalytic processing (2). After the autocleavage in the ER, the pro domain and mature chain exit the cell together through non-covalent interactions (3). PCSK9 is a key regulator of LDL-cholesterol levels (LDL-C) through binding of the LDL receptor, resulting in the reduction of receptor recycling to the cell surface and the acceleration of receptor degradation in lysosomes (3). Both gain of function (GOF) and loss-of-function (LOF) mutations have been found in the PCSK9 gene (3). GOF mutations are linked to familial autosomal dominant hypercholesterolemia, a disease characterized by elevated plasma levels of LDL-C. In comparison, LOF mutations lead to low levels of LDL-C and protection against coronary heart disease.

References

  1. Seidah, N.G. et al. (2003) Proc. Natl. Acad. Sci. USA 100:928.
  2. Naureckiene, S. et al. (2003) Arch. Biochem. Biophys. 420:55.
  3. Costet, P. et al. (2008) Trends Biochem. Sci. 33:426.

Alternate Names

FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, PCSK9

Entrez Gene IDs

255738 (Human); 100102 (Mouse); 298296 (Rat); 102142788 (Cynomolgus Monkey)

Gene Symbol

PCSK9

UniProt

Q8NBP7

Additional Proprotein Convertase 9/PCSK9 Products

Product Documents for Human Proprotein Convertase 9/PCSK9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Proprotein Convertase 9/PCSK9 Antibody

For research use only

Related Research Areas

Obesity
Serine Proteases and Regulators

Citations for Human Proprotein Convertase 9/PCSK9 Antibody

Customer Reviews for Human Proprotein Convertase 9/PCSK9 Antibody (6)

4 out of 5
6 Customer Ratings
5 Stars
17%
4 Stars
67%
3 Stars
17%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 6 reviews Showing All
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  • Human Proprotein Convertase 9/PCSK9 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: HepG2 human hepatocellular carcinoma cell line
    Species: Human
    Verified Customer | Posted 07/25/2024
    Human Proprotein Convertase 9/PCSK9 Antibody AF3888
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: hPCKS9 in transfected HepG2 cells
    Species: Human
    Verified Customer | Posted 12/18/2014
  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: IP of human PCSK9
    Species: Human
    Verified Customer | Posted 12/18/2014
  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Recombinant hPCSK9 expressed in 293 and HepG2 cells
    Species: Human
    Verified Customer | Posted 12/18/2014
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Cells transfected with hPCSK9 cDNA
    Species: Human
    Verified Customer | Posted 12/18/2014
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant hPCSK9 expressed in 293 and HepG2 cells
    Species: Human
    Verified Customer | Posted 12/18/2014

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