Human Prostatic Acid Phosphatase/ACPP Antibody
Human Prostatic Acid Phosphatase/ACPP Antibody Summary
Met1-Gln379
Accession # P15309
Applications
This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Human Prostatic Acid Phosphatase/ACPP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6240).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Prostatic Acid Phosphatase/ACPP DuoSet ELISA Kit (Catalog # DY6240-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Human Prostatic Acid Phosphatase/ACPP ELISA Standard Curve. Recombinant Human Prostatic Acid Phosphatase/ACPP protein was serially diluted 2-fold and captured by Mouse Anti-Human Prostatic Acid Phosphatase/ACPP Monoclonal Antibody (Catalog # MAB62401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Prostatic Acid Phosphatase/ACPP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6240) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Prostatic Acid Phosphatase/ACPP
Prostatic Acid Phosphatase (ACPP) catalyzes the hydrolysis of a variety of phosphate monoesters, including phosphorylated proteins (1). The activity optimum of ACPP is in the pH range of 4 - 6, and the activity is inhibited by L(+)-tartrate. ACPP expression levels are highest in the prostate, with much lower expression in most other tissues. ACPP is a type I integral membrane protein of the plasma membrane and lysosomes, and a secreted form also exists (2). The concentration of ACPP is elevated in the circulation of prostate cancer patients, making the enzyme a marker for the progression of prostate cancer (3). Cellular ACPP has been shown to be a protein tyrosine phosphatase (4). Protein substrates include the epidermal growth factor receptor and HER-2 (5). Cellular ACPP is considered to function as a tumor suppressor (5).
- Apostol, I. et al. (1985) Acta. Biochim. Pol. 32:187.
- Schroeder, B. et al. (2007) Traffic 8:1676.
- Gutman, E.B. et al. (1936) Am. J. Cancer 28:485.
- Lin, M.F. and G.M. Clinton (1986) Biochem. J. 235:351.
- Veeramani, S. et al. (2005) Endocr. Relat. Cancer 12:805.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Prostatic Acid Phosphatase/ACPP Antibody
There are currently no reviews for this product. Be the first to review Human Prostatic Acid Phosphatase/ACPP Antibody and earn rewards!
Have you used Human Prostatic Acid Phosphatase/ACPP Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
