Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 911603R
View all Prostatic Acid Phosphatase/ACPP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Prostatic Acid Phosphatase/ACPP
Met1-Gln379
Accession # P15309

Specificity

Detects human Prostatic Acid Phosphatase/ACPP in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Prostatic Acid Phosphatase/ACPP Antibody

Human Prostatic Acid Phosphatase/ACPP Antibody in ELISA Standard Curve.

Human Prostatic Acid Phosphatase/ACPP ELISA Standard Curve.

Recombinant Human Prostatic Acid Phosphatase/ACPP protein was serially diluted 2-fold and captured by Mouse Anti-Human Prostatic Acid Phosphatase/ACPP Monoclonal Antibody (Catalog # MAB62401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Prostatic Acid Phosphatase/ACPP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6240) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human Prostatic Acid Phosphatase/ACPP Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Human Prostatic Acid Phosphatase/ACPP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6240).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Prostatic Acid Phosphatase/ACPP DuoSet ELISA Kit (Catalog # DY6240-05) for convenient development of a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Prostatic Acid Phosphatase/ACPP

Prostatic Acid Phosphatase (ACPP) catalyzes the hydrolysis of a variety of phosphate monoesters, including phosphorylated proteins (1). The activity optimum of ACPP is in the pH range of 4 - 6, and the activity is inhibited by L(+)-tartrate. ACPP expression levels are highest in the prostate, with much lower expression in most other tissues. ACPP is a type I integral membrane protein of the plasma membrane and lysosomes, and a secreted form also exists (2). The concentration of ACPP is elevated in the circulation of prostate cancer patients, making the enzyme a marker for the progression of prostate cancer (3). Cellular ACPP has been shown to be a protein tyrosine phosphatase (4). Protein substrates include the epidermal growth factor receptor and HER-2 (5). Cellular ACPP is considered to function as a tumor suppressor (5).

References

  1. Apostol, I. et al. (1985) Acta. Biochim. Pol. 32:187.
  2. Schroeder, B. et al. (2007) Traffic 8:1676.
  3. Gutman, E.B. et al. (1936) Am. J. Cancer 28:485.
  4. Lin, M.F. and G.M. Clinton (1986) Biochem. J. 235:351.
  5. Veeramani, S. et al. (2005) Endocr. Relat. Cancer 12:805.

Alternate Names

ACP3, ACPP

Entrez Gene IDs

55 (Human); 563181 (Mouse); 56780 (Rat)

Gene Symbol

ACP3

UniProt

P15309 (Human)

Additional Prostatic Acid Phosphatase/ACPP Products

Product Documents for Human Prostatic Acid Phosphatase/ACPP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Prostatic Acid Phosphatase/ACPP Antibody

For research use only

Related Research Areas

Phosphatases and Regulators

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