Human PTGFR Antibody

Catalog # Availability Size / Price Qty
MAB10294-100
MAB10294-SP
Human PTGFR Antibody in Flow Cytometry
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Human PTGFR Antibody Summary

Species Reactivity
Human
Specificity
Detects human PTGFR in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 1014602
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide containing human PTGFR RD3 epitope
Accession # P43088
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of PTGFR in HEK293 Human Cell Line Transfected with Human PTGFR and eGFP by Flow Cytometry View Larger

Detection of PTGFR in HEK293 Human Cell Line Transfected with Human PTGFR and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with (A) human PTGFR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human PTGFR Monoclonal Antibody (Catalog # MAB10294) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTGFR

The prostaglandin F2-alpha receptor (or PTGR) is a member of the Prostanoid Receptor subfamily and a member of the G-protein coupled receptor (GPCR) family. Along with various other prostaglandin receptors, Prostaglandin F Receptor mediates the effects of prostaglandin F2 alpha (PGF2 alpha ). Alternative mRNA splicing gives rise to many isoforms; Isoforms 2 to 7 do not bind PGF2-alpha but are proposed to modulate signaling by participating in variant receptor complexes. Some of these isoforms have been proposed to form heterodimers.

Long Name
Prostaglandin F Receptor
Entrez Gene IDs
5737 (Human); 19220 (Mouse); 25652 (Rat)
Alternate Names
FP; MGC120498; MGC46203; PGF receptor; PGF2 alpha receptor; PGF2-alpha Receptor; prostaglandin F receptor (FP); prostaglandin F2 alpha receptor; prostaglandin F2-alpha receptor; prostaglandin receptor (2-alpha); Prostanoid FP receptor; PTGFR

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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