Human/Rat Brevican Antibody

  (2 citations)
(1 Review)
    
Datasheet / CoA / SDS
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Human, Rat
  • Specificity
    Detects human Brevican in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Brevican is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Brevican
    Asp23-Pro911
    Accession # AAH27971
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Brevican by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue and human brain (motor cortex) tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Rat Brevican Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4009) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Brevican at approximately 60 and 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Brevican in Rat Cortical Stem Cells. Brevican was detected in immersion fixed rat cortical stem cells differentiated for 7 days by growth factor withdrawal using Sheep Anti-Human/Rat Brevican Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4009) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Immunohistochemistry
Brevican in Human Brain. Brevican was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human/Rat Brevican Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4009) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuropil. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Brevican
Brevican, also called BEHAB, is a secreted member of the the lectican family of proteoglycans that share a common domain structure (1). Brevican contains an Ig‑like V-set domain, two link domains, a Glu-rich region, a central region with glycosaminoglycan (GAG) modifications, an EGF-like domain, a C-type lectin domain, and a C-terminal Sushi/CRP-like domain (2). Brevican is restricted to the CNS and is expressed by astrocytes, oligodendrocytes, and neurons (3‑7). A GPI-anchored alternate splice form exists that is truncated following the central (GAG) region (2, 8). Brevican is cleaved by multiple proteases and exists in a number of distinct fragments (5, 9, 10). Full-length brevican consists of a 97 kDa core protein with up to approximately 100 kDa of attached chondroitin sulfate but not heparan sulfate chains (4, 7, 11, 12). Brevican associates with the extracellular matrix, perineuronal nets, and astrocyte cell surfaces by means of its chondroitin sulfate, GPI anchor, hyaluronic acid-binding link domains, and the core protein (4, 7, 8, 13). The secreted isoform is dominant during brain development and is up‑regulated in astrocytes following brain injury (2, 14). In human and rat, an under-glycosylated form of brevican is up‑regulated in highly aggressive glioma but not in low-grade glioma or other brain pathologies (15, 16). In mouse and rat, levels of an ADAMTS4-generated 55 kDa N-terminal fragment increase during remodeling after excitotoxic injury (11, 12). Human brevican shares 90%, 80%, and 80% aa sequence identity with bovine, mouse, and rat brevican, respectively. Within the Ig-like and two link domains, brevican shares 45%‑51% aa sequence identity with aggrecan, neurocan, and versican.
  • References:
    1. Viapiano, M.S. and R.T. Matthews (2006) Trends Mol. Med. 12:488. 
    2. Gary, S.C. et al. (2000) Gene 256:139. 
    3. Jaworski, D.M. et al. (1994) J. Cell Biol. 125:495. 
    4. Seidenbecher, C.I. et al. (1995) J. Biol. Chem. 270:27206. 
    5. Hamel, M.G. et al. (2005) J. Neurochem. 93:1533. 
    6. Ogawa, T. et al. (2001) J. Comp. Neurol. 432:285  
    7. Yamada, H. et al. (1997) J. Neurosci. 17:7784.  
    8. Seidenbecher, C.I. et al. (2002) J. Neurochem. 83:738.
    9. Matthews, R.T. et al. (2000) J. Biol. Chem. 275:22695.
    10. Nakamura, H. et al. (2000) J. Biol. Chem. 275:38885.
    11. Mayer, J. et al. (2005) BMC Neurosci. 6:52.
    12. Yuan, W. et al. (2002) Neuroscience 114:1091.
    13. Deepa, S.S. et al. (2006) J. Biol. Chem. 281:17789.
    14. Jaworski, D.M. et al. (1999) Exp. Neurol. 157:327.
    15. Viapiano, M.S. et al. (2005) Cancer Res. 65:6726.
    16. Viapiano, M.S. et al. (2003) J. Biol. Chem. 278:33239.
  • Long Name:
    Brain Specific Proteoglycan in the Aggrecan Family
  • Entrez Gene IDs:
    63827 (Human); 12032 (Mouse); 25393 (Rat)
  • Alternate Names:
    ALPBRE; BCAN; BEHAB; BEHABbrevican core protein; Brain-enriched hyaluronan-binding protein; Brevican; Chondroitin sulfate proteoglycan 7; chondroitin sulfate proteoglycan BEHAB; chondroitin sulfate proteoglycan BEHAB/brevican; CSPG7; CSPG7brevican proteoglycan; MGC13038
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Species
Applications
Sample Type
  1. Perineuronal Nets in Spinal Motoneurones: Chondroitin Sulphate Proteoglycan around Alpha Motoneurones
    Authors: SF Irvine, JCF Kwok
    Int J Mol Sci, 2018;19(4):.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  2. Sensory experience-dependent formation of perineuronal nets and expression of Cat-315 immunoreactive components in the mouse somatosensory cortex
    Authors: H Ueno, S Suemitsu, M Okamoto, Y Matsumoto, T Ishihara
    Neuroscience, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-Fr

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

CTS019 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
ExactaChIP Chromatin Immunoprecipitation Kits
Description Application Cat# Citations Images  

ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 11
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB , Simple Western HAF016 18  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL010 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL011 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL012 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 1 review)

Have you used Human/Rat Brevican Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Human/Rat Brevican Antibody.

We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

Filter by Ratings
Excellent
(1)
All Ratings
Filter by Applications
ICC/IF
(1)
All Applications
Filter by Species
Rat
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human/Rat Brevican Antibody AF4009 Array AF4009
Enlarge
Excellent
 ICC/IF Rat Anonymous 04/10/2018
Show
Image Details
Immunocytochemistry/Immunofluorescence Human/Rat Brevican Antibody AF4009
Immunocytochemistry/Immunofluorescence: Human/Rat Brevican Antibody [AF4009].

Summary

Application Immunocytochemistry/Immunofluorescence
Sample Tested Adult brain
Species Rat

Other Experimental Details

Other Experimental Details Used at a 1:500 but will need to increase to 1:100 for better staining

Please scroll the above reviews for more

Order Details

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies
R&D Systems Guarantee