Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1279D
View all SUSD2 Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line-derived human SUSD2
Met1-Ala785
Accession # Q9UGT4

Specificity

Detects human SUSD2 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Rat SUSD2 Antibody

Detection of SUSD2 antibody by Western Blot.

Detection of SUSD2 by Western Blot.

Western blot shows lysates of SK-BR-3 human breast cancer cell line, human lung tissue, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Rat SUSD2 Monoclonal Antibody (Catalog # MAB90563) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for SUSD2 at approximately 110 and 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of SUSD2 antibody in HEK293 Human Cell Line Transfected with Human SUSD2 and eGFP antibody by Flow Cytometry.

Detection of SUSD2 in HEK293 Human Cell Line Transfected with Human SUSD2 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human SUSD2 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human/Rat SUSD2 Monoclonal Antibody (Catalog # MAB90563) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Rat SUSD2 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human SUSD2 and eGFP

Western Blot

1 µg/mL
Sample: SK‑BR‑3 human breast cancer cell line, human lung tissue, and rat kidney tissue

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SUSD2

Sushi domain containing 2, or SUSD2, is a type I transmembrane protein of 822 amino acids containing functional domains inherent to adhesion molecules. SUSD2 has been described as a novel marker of human endometrial mesenchymal stem-like cells and it has been used for their prospective Isolation. As a transmembrane receptor, SUSD2 has been proposed to interact with Galectin-1 and to be the receptor for C10ORF99, a novel potential cytokine suggested to inhibits colon cancer cell growth through inducing G1 arrest. There is  evidence that SUSD2 may play a role in breast tumorigenesis.

Long Name

Sushi Domain Containing 2

Alternate Names

BK65A6.2

Entrez Gene IDs

56241 (Human); 71733 (Mouse); 294335 (Rat)

Gene Symbol

SUSD2

UniProt

Q9UGT4

Additional SUSD2 Products

Product Documents for Human/Rat SUSD2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Rat SUSD2 Antibody

For research use only

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