Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1090828
View all RBPMS Primary Antibodies »

Product Specifications

Immunogen

Synthetic Peptide
Accession # Q93062

Specificity

Detects a peptide specific for human RBPMS around amino acid 150 in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human RBPMS Antibody

Detection of Human RBPMS by Western Blot.

Western Blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line and JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human RBPMS Monoclonal Antibody (Catalog # MAB11670) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for RBPMS at approximately 28, 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of RBPMS in Human Testis.

RBPMS was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human RBPMS Monoclonal Antibody (Catalog # MAB11670) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm of smooth muscle. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human RBPMS Antibody

Application
Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testis

Western Blot

2 µg/mL
Sample: A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line and JEG-3 human epithelial choriocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RBPMS

RBPMS is a 22kDa protein that is a member of the RRM family of RNA-binding proteins.  RBPMS has been identified as a critical splicing regulator in differentiated vascular smooth muscle cells.  RBPMS is expressed at high levels in the heart, breasts, lungs, kidneys, stomach, muscles, liver, eyes, adipose tissue, and ovaries.  Studies in breast cancer and ovarian cancer show that RBPMS has a potential role as a tumor suppressor.

References

  1. Nakagaki-Silva EE, Gooding C, Llorian M, Jacob AG, Richards F, Buckroyd A, Sinha S, Smith CWJ. Identification of RBPMS as a mammalian smooth muscle master splicing regulator via proximity of its gene with super-enhancers. Elife. 2019 Jul 8;8:e46327. doi: 10.7554/eLife.46327. PMID: 31283468; PMCID: PMC6613909. 
  2. Rabelo-Fernández RJ, Santiago-Sánchez GS, Sharma RK, Roche-Lima A, Carrion KC, Rivera RAN, Quiñones-Díaz BI, Rajasekaran S, Siddiqui J, Miles W, Rivera YS, Valiyeva F, Vivas-Mejia PE. Reduced RBPMS Levels Promote Cell Proliferation and Decrease Cisplatin Sensitivity in Ovarian Cancer Cells. Int J Mol Sci. 2022 Jan 4;23(1):535. doi: 10.3390/ijms23010535. PMID: 35008958; PMCID: PMC8745614.

Long Name

RNA binding protein, mRNA processing factor

Alternate Names

HERMES, RBPMS

Entrez Gene IDs

11030 (Human); 19663 (Mouse); 498642 (Rat)

Gene Symbol

RBPMS

UniProt

Q93062 (Human)

Additional RBPMS Products

Product Documents for Human RBPMS Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human RBPMS Antibody

For research use only

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Protocols

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