Human RBPMS Antibody

Catalog #: MAB11670 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11670-100
MAB11670-SP

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Detection of Human RBPMS by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RBPMS Antibody Summary

Species Reactivity

Human

Specificity

Detects a peptide specific for human RBPMS around amino acid 150 in Direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1090828

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic Peptide
Accession # Q93062

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line and JEG-3 human epithelial choriocarcinoma cell line

Immunohistochemistry

3-25 µg/mL

Immersion fixed paraffin-embedded sections of human testis

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human RBPMS by Western Blot. Western Blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line and JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human RBPMS Monoclonal Antibody (Catalog # MAB11670) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for RBPMS at approximately 28, 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunohistochemistry

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Detection of RBPMS in Human Testis. RBPMS was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human RBPMS Monoclonal Antibody (Catalog # MAB11670) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm of smooth muscle. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RBPMS

RBPMS is a 22kDa protein that is a member of the RRM family of RNA-binding proteins. RBPMS has been identified as a critical splicing regulator in differentiated vascular smooth muscle cells. RBPMS is expressed at high levels in the heart, breasts, lungs, kidneys, stomach, muscles, liver, eyes, adipose tissue, and ovaries. Studies in breast cancer and ovarian cancer show that RBPMS has a potential role as a tumor suppressor.

References

Nakagaki-Silva EE, Gooding C, Llorian M, Jacob AG, Richards F, Buckroyd A, Sinha S, Smith CWJ. Identification of RBPMS as a mammalian smooth muscle master splicing regulator via proximity of its gene with super-enhancers. Elife. 2019 Jul 8;8:e46327. doi: 10.7554/eLife.46327. PMID: 31283468; PMCID: PMC6613909.
Rabelo-Fernández RJ, Santiago-Sánchez GS, Sharma RK, Roche-Lima A, Carrion KC, Rivera RAN, Quiñones-Díaz BI, Rajasekaran S, Siddiqui J, Miles W, Rivera YS, Valiyeva F, Vivas-Mejia PE. Reduced RBPMS Levels Promote Cell Proliferation and Decrease Cisplatin Sensitivity in Ovarian Cancer Cells. Int J Mol Sci. 2022 Jan 4;23(1):535. doi: 10.3390/ijms23010535. PMID: 35008958; PMCID: PMC8745614.

Long Name

RNA binding protein, mRNA processing factor

Entrez Gene IDs

11030 (Human); 19663 (Mouse); 498642 (Rat)

Alternate Names

FLJ32971; Heart and RRM expressed sequence; HERMES; HERMESRNA-binding protein with multiple splicing; RBPMS; RBP-MS; RNA binding protein with multiple splicing