Detects human Reg1A in ELISAs. In direct ELISAs, approximately 20% - 50%
cross-reactivity with recombinant human (rh) Reg1B is observed and no
cross-reactivity with rhReg3A, recombinant mouse Reg1, or recombinant rat Reg1A
Monoclonal Rat IgG2A Clone # 431211
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Reg1A Gln23-Asn166 Accession # P05451
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Recombinant Human Reg1A
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reg1A in Human Pancreas. Reg1A was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human Reg1A Monoclonal Antibody (Catalog # MAB49371) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Reg1A, also known as PTP, PSP, and lithostathine, is a member of the Reg family of secreted proteins with a C-type lectin domain. Due to variable glycosylation, pancreatic Reg1A exists as multiple species of 16‑18 kDa. Reg1A promotes the maintenance and growth of pancreatic islet beta -cells and intestinal villi. It is up‑regulated in pancreatitis and some carcinomas. Reg1A is an antigenic target in autoimmune diabetes. Human Reg1A shares 65%‑68% aa sequence identity with mouse and rat Reg1A.
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