Human Reg1A Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Reg1A in ELISAs. In direct ELISAs, approximately 20% - 50% cross-reactivity with recombinant human (rh) Reg1B is observed and no cross-reactivity with rhReg3A, recombinant mouse Reg1, or recombinant rat Reg1A is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 431211
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Reg1A 
    Gln23-Asn166
    Accession # P05451
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human Reg1A
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Reg1A in Human Pancreas. Reg1A was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human Reg1A Monoclonal Antibody (Catalog # MAB49371) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Reg1A

Reg1A, also known as PTP, PSP, and lithostathine, is a member of the Reg family of secreted proteins with a C-type lectin domain. Due to variable glycosylation, pancreatic Reg1A exists as multiple species of 16‑18 kDa. Reg1A promotes the maintenance and growth of pancreatic islet beta -cells and intestinal villi. It is up‑regulated in pancreatitis and some carcinomas. Reg1A is an antigenic target in autoimmune diabetes. Human Reg1A shares 65%‑68% aa sequence identity with mouse and rat Reg1A.

  • Long Name:
    Regenerating Islet-derived 1A
  • Entrez Gene IDs:
    5967 (Human)
  • Alternate Names:
    ICRF; PSP; PTP; Reg1A
Related Research Areas
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Rat HRP-DAB Cell & Tissue Staining Kit

CTS017 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control

Ctrl 6-001-A 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2A Isotype Control

Ctrl MAB006R  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Antibody

WB AF005 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human Reg1A Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information