Detects human Reg1A in direct ELISAs. In direct ELISAs, approximately 80% cross-reactivity with recombinant human (rh) Reg1B is observed, approximately 20% cross-reactivity with recombinant mouse Reg1, and less than 5% cross-reactivity with rhReg3A and recombinant rat Reg1A is observed.
Polyclonal Sheep IgG
Chinese hamster ovary cell line CHO-derived recombinant human Reg1A Gln23-Asn166 Accession # P05451
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human Reg1A by Western Blot.
Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Reg1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4937) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Reg1A at approximately 20-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reg1A in Human Pancreas.
Reg1A was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human Reg1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4937) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Reg1A (Regenerating gene IA/ alpha ; also REG, ICRF, PSPS, PTP and Lithostathine-1 alpha ) is a secreted, variably glycosylated 15-22 kDa type I member of the REG gene family of proteins. It is induced in proliferating cell types such as colonic epithelium, islet beta -cells, and multiple tumor types, and is constitutively expressed by pancreatic acinar cells. Thus, Reg1A is found both in blood (ng/mL quantities) and pancreatic secretions. It would appear that Reg1A plays a role in blocking apoptosis, and is upregulated by both IL-22 and IL-6. Human Reg1A is synthesized as a 166 amino acid (aa) precursor. It contains a 22 aa signal sequence plus a 144 aa mature region that is characterized by the presence of three intrachain disulfide bonds, one C-type lectin domain (aa 34-164), and a utilized N-terminal O-linked glycosylation site (Thr27). Variablility in the glycosylation pattern, plus proteolytic cleavage after Arg11 generates Reg1A isoforms in the 15-22 kDa range. There is one potential isoform variant that shows an alternative start site at Met9. Mature (aa 23-166) human Reg1A shares 76% and 69% aa sequence identity with mouse and rat Reg1A, respectively.
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