Human Rev‑erb beta /NR1D2 Antibody

R&D Systems | Catalog # PP-H2729-00

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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Direct ELISA, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # H2729
View all Rev-erb beta/NR1D2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Rev-erb beta/NR1D2
aa 4-101

Specificity

This antibody specifically recognizes human Rev-Erb beta and cross-reacts with rat Rev-Erb beta. It does not recognize human Rev-Erb alpha. Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Applications for Human Rev‑erb beta /NR1D2 Antibody

Application
Recommended Usage

Direct ELISA

This antibody can be used at 0.3 μg/mL with the appropriate secondary reagents to detect human Rev-Erb beta.

Immunohistochemistry

This antibody can be used at 50 - 100 μg/mL with the appropriate secondary reagents to detect human Rev-Erb beta.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Western Blot

This antibody can be used at 1 μg/mL under reducing conditions with the appropriate secondary reagents to detect human Rev-Erb beta.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

A liquid formulation in physiologic saline with 0.1% NaN3.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: Rev-erb beta/NR1D2

Reverse Orientation the c-ErbA-1 Gene beta (Rev-Erb beta, BD73; NR1D2) is a member of the Orphan Nuclear Receptor family. Rev-Erb beta is expressed in the heart, brain, lung, liver, skeletal muscles, kidney, spleen and testis. Rev-Erb beta plays a broad role in cell proliferation and physiology.

Alternate Names

BD73, EAR-1R, NR1D2, Reverb beta

Entrez Gene IDs

9975 (Human)

Gene Symbol

NR1D2

Additional Rev-erb beta/NR1D2 Products

Product Documents for Human Rev‑erb beta /NR1D2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Rev‑erb beta /NR1D2 Antibody

For research use only

Citations for Human Rev‑erb beta /NR1D2 Antibody

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Protocols

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FAQs

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