Human RIPK3/RIP3 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB76041-100
MAB76041-SP
Detection  of Human RIPK3/RIP3 by Western Blot.
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Human RIPK3/RIP3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human RIPK3/RIP3 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1312A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Synthetic peptide containing human RIPK3/RIP3
Accession # Q9Y572
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Human RIPK3/RIP3 by Western Blot. View Larger

Detection of Human RIPK3/RIP3 by Western Blot. Western blot shows lysates of SK‑BR‑3 human breast cancer cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human RIPK3/RIP3 Monoclonal Antibody (Catalog # MAB76041) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for RIPK3/RIP3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RIPK3/RIP3

RIP3 (Receptor-Interacting Protein 3), also known as RIPK3, is a 518 amino acid (aa), 60 kDa phosphoprotein that contains an N-terminal protein kinase domain and a C-terminal interaction motif with which it binds RIP1. It converts cell response to TNF-a from apoptosis (RIP1 via Fas) to programmed necrosis (RIP1/RIP3) by inducing reactive oxygen species production. Human RIP3 (aa 1-218) shares approximately 74% aa sequence identity with mouse and rat RIP3. Alternately spliced human b (252 aa) and g (231 aa) isoforms diverge after aa 219 and 221, respectively, and are found to antagonize RIP3.

Long Name
Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 3
Entrez Gene IDs
11035 (Human); 56532 (Mouse); 246240 (Rat)
Alternate Names
EC 2.7.11.1; Receptor-interacting protein 3; receptor-interacting serine/threonine-protein kinase 3; receptor-interacting serine-threonine kinase 3; RIP3; RIP-3; RIP3receptor interacting protein 3; RIPK3; RIP-like protein kinase 3

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