Human Rit1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Rit1 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 456201
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Rit1
    Met1-Thr219
    Accession # Q92963
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Rit1 by Western Blot. Western blot shows lysates of human brain tissue and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Rit1 Monoclonal Antibody (Catalog # MAB4697) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Rit1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rit1

Rit1 is a member of the Ras-related small GTPase superfamily. The 219 amino acid, 25 kDa human Rit1 is a cytoplasmic cell membrane-associated protein that is expressed in most tissues. It is widely expressed in the developing and adult nervous systems and participates in NGF-induced axon outgrowth. Rit1 forms complexes with other small GTPases, such as cdc42 and Rac1, that are important during cell growth and polarity. Human Rit1 shares 95% amino acid identity with mouse and rat Rit1.

  • Long Name:
    Ras-like without CAAX 1
  • Entrez Gene IDs:
    6016 (Human); 19769 (Mouse); 499652 (Rat)
  • Alternate Names:
    GTP-binding protein Rit1; GTP-binding protein Roc1; MGC125864; MGC125865; Ras-like protein expressed in many tissues; Ras-like without CAAX 1; Ras-like without CAAX protein 1; RIBB; RIBBRIT; Ric-like, expressed in many tissues; RIT; Rit1; ROC1; ROC1Ric (Drosophila)-like, expressed in many tissues
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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