Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 456201
View all Rit1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Rit1
Met1-Thr219
Accession # Q92963

Specificity

Detects human Rit1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Rit1 Antibody

Detection of Human Rit1 antibody by Western Blot.

Detection of Human Rit1 by Western Blot.

Western blot shows lysates of human brain tissue and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Rit1 Monoclonal Antibody (Catalog # MAB4697) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Rit1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human Rit1 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human brain tissue and HUVEC human umbilical vein endothelial cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Rit1

Rit1 is a member of the Ras-related small GTPase superfamily. The 219 amino acid, 25 kDa human Rit1 is a cytoplasmic cell membrane-associated protein that is expressed in most tissues. It is widely expressed in the developing and adult nervous systems and participates in NGF-induced axon outgrowth. Rit1 forms complexes with other small GTPases, such as cdc42 and Rac1, that are important during cell growth and polarity. Human Rit1 shares 95% amino acid identity with mouse and rat Rit1.

Long Name

Ras-like without CAAX 1

Alternate Names

RIBB, ROC1

Entrez Gene IDs

6016 (Human); 19769 (Mouse); 499652 (Rat)

Gene Symbol

RIT1

UniProt

Q92963

Additional Rit1 Products

Product Documents for Human Rit1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Rit1 Antibody

For research use only

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