Human ROBO2 Antibody

Catalog # Availability Size / Price Qty
AF3147
AF3147-SP
Product Details
Citations (6)
FAQs
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Human ROBO2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ROBO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant rat ROBO1, recombinant human (rh) ROBO3 and rhROBO4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ROBO2
Ser22-Ala312, RPVA, Pro313-Pro859
Accession # Q9HCK4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ROBO2 Fc Chimera (Catalog # 3147-RB)
Flow Cytometry
2.5 µg/106 cells
NTera‑2 human testicular embryonic carcinoma cell line differentiated with retinoic acid
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
Immersion fixed human neural progenitor cells differentiated by growth factor withdrawal

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROBO2

Human ROBO2 is a 175 kDa member of the ROBO/roundabout receptor family and is a 1357 amino acid (aa) type I transmembrane (TM) protein. It contains an extracellular domain (ECD) comprised of five C2-type Ig-like domains and three fibronectin type III domains. ROBO receptors interact with SLIT proteins to regulate axon guidance. Similar to ROBO1, the first two Ig-like domains of ROBO2 are predicted to be important for SLIT binding. Alternate splice forms of ROBO2 exist. The ROBO2 ECD in human shares 98% aa identity with the ROBO2 ECD in mouse and canine.

Long Name
Roundabout Homolog 2
Entrez Gene IDs
6092 (Human); 268902 (Mouse); 84409 (Rat)
Alternate Names
Drosophila) homolog 2; ROBO2; roundabout, axon guidance receptor, homolog 2 (Drosophila); SAX3

Product Datasheets

Citations for Human ROBO2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Ndfip Proteins Target Robo Receptors for Degradation and Allow Commissural Axons to Cross the Midline in the Developing Spinal Cord
    Authors: M Gorla, C Santiago, K Chaudhari, AAK Layman, PM Oliver, GJ Bashaw
    Cell Rep, 2019;26(12):3298-3312.e4.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  2. Sequential arrival and graded secretion of Sema3F by olfactory neuron axons specify map topography at the bulb.
    Authors: Takeuchi H, Inokuchi K, Aoki M
    Cell, 2010;141(6):1056-67.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. The chemorepellent Slit3 promotes monocyte migration.
    Authors: Geutskens SB, Hordijk PL, van Hennik PB
    J. Immunol., 2010;185(12):7691-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Manipulating Robo expression in vivo perturbs commissural axon pathfinding in the chick spinal cord.
    Authors: Reeber SL, Sakai N, Nakada Y, Dumas J, Dobrenis K, Johnson JE, Kaprielian Z
    J. Neurosci., 2008;28(35):8698-708.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Robos and slits control the pathfinding and targeting of mouse olfactory sensory axons.
    Authors: Nguyen-Ba-Charvet KT, Di Meglio T, Fouquet C, Chedotal A
    J. Neurosci., 2008;28(16):4244-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. The role of Slit-Robo signaling in the generation, migration and morphological differentiation of cortical interneurons.
    Authors: Andrews W, Barber M, Hernadez-Miranda LR, Xian J, Rakic S, Sundaresan V, Rabbitts TH, Pannell R, Rabbitts P, Thompson H, Erskine L, Murakami F, Parnavelas JG
    Dev. Biol., 2007;313(2):648-58.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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