Human SARM1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human SARM1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human SARM1
    Lys556-Thr724
    Accession # Q6SZW1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    SH‑SY5Y human neuroblastoma cell line
  • Immunoprecipitation
    4 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunoprecipitation

Immunoprecipitation of Human SARM1. SARM1 was immunoprecipitated from 400 µg of SH‑SY5Y human neuroblastoma cell line lysates using 4 µg of Sheep Anti-Human SARM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7037) coated on 4 wells of a 96 well plate (Corning Costar EIA/RIA). SH‑SY5Y lysates or control buffer were added to the wells and incubated for 2 hours at room temperature. Immunoprecipitated SARM1 was detected by Western blot under reducing conditions using 1.0 µg/mL Sheep Anti-Human SARM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7037) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation.

Immunocytochemistry

SARM1 in HEK293 Human Cell Line. SARM1 was detected in immersion fixed HEK293 human embryonic kidney cell line using Sheep Anti-Human SARM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7037) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SARM1

SARM1 (Sterile alpha and TIR motif-containing protein 1; also SAM domain-containing protein 2 and TIR-1 homolog) is a 73-75 kDa member of the TLR adaptor family of molecules.  It is both nuclear and cytoplasmic, and expressed in monocytes, neurons, and retinal photoreceptor cells, where it demonstrates disparate activities.  In monocytes, SARM1 is a specific inhibitor of TRIF-dependent TLR-3 and -4 signaling, and appears to block MAPK phosphorylation.  In neurons, SARM1 regulates microtubule stability, and thus axon and dendrite elongation.  And in retinal photoreceptor cells, SARM1 complexes with Na/K ATPase to create a cell surface receptor for retinoschisin.  Human SARM1 is 724 amino acids (aa) in length.  It contains an N-terminal polybasic motif (aa 1-20) and a Gly-rich region (aa 22-90) that may anchor the molecule to intracellular membranes.  It also possesses two SAM domains (aa 412-548) plus a TIR region (aa 559-657) that appears to interact with TIRF.  Proteolytic cleavage products of 35 kDa and 30 kDa have been described.  There is one splice variant that shows a 72 aa substitution for aa 1-106.  Over aa 556-724, human SARM1 shares 94% aa identity with mouse SARM1.    

  • Long Name:
    Sterile alpha and TIR Motif Containing 1
  • Entrez Gene IDs:
    23098 (Human); 237868 (Mouse); 287545 (Rat)
  • Alternate Names:
    KIAA0524SAM domain-containing protein 2; SAMD2; SAMD2sterile alpha and HEAT/Armadillo motif protein, ortholog of Drosophila; SARM1; SARMFLJ36296; Sterile alpha and Armadillo repeat protein; sterile alpha and TIR motif containing 1; sterile alpha and TIR motif-containing protein 1; Sterile alpha motif domain-containing protein 2; Tir-1 homolog
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Sheep HRP-DAB Cell & Tissue Staining Kit

CTS019 1
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ExactaChIP Chromatin Immunoprecipitation Kits
Description Application Cat# Citations Images  

ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
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Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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