Semaphorin 3E (Sema3E) is a secreted protein that functions in axon guidance and/or vascular tip cell guidance during development. Sema3E signals through Plexin D1 that may also be associated with Neuropilin 1 and/or VEGF R2. It can exert axon repulsive or attraction effects, promote axonal growth, the development of glutamatertic synaptic development, and the development of GnRH producing neurons. Sema3E also plays a role in the regulation of vascular growth and branching. It inhibits smooth muscle cell proliferation and migration in the asthmatic airway, promotes hepatic stellate cell activation and wound healing, and regulates the migration of developing thymocytes. Sema3E is upregulated by inflammatory macrophages and damaged hepatocytes. It can be cleaved to generate a form that does not dimerize and is highly expressed in tumor cell lines with metastatic potential.
Human Semaphorin 3E DuoSet ELISA
R&D Systems | Catalog # DY3239-05
Key Product Details
Assay Type
Assay Range
Sample Type
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human Semaphorin 3E DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human Semaphorin 3E DuoSet ELISA
Product Specifications
Assay Format
Sample Volume Required
Detection Method
Conjugate
Specificity
Label
Scientific Data Images for Human Semaphorin 3E DuoSet ELISA
Human Semaphorin 3E ELISA Standard Curve
Kit Contents for Human Semaphorin 3E DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS:
(Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .
Preparation and Storage
Shipping
Stability & Storage
Background: Semaphorin 3E
Additional Semaphorin 3E Products
Product Documents for Human Semaphorin 3E DuoSet ELISA
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Semaphorin 3E DuoSet ELISA
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
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- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars