Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Semaphorin 4C Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4C
Ala21-Gly663
Accession # Q9C0C4

Specificity

Detects human Semaphorin 4C in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse (rm) Sema4C is observed and less than 5% cross-reactivity with recombinant human (rh) Sema4G, rhSema4D, rhSema4B and rhSema4A is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Semaphorin 4C Antibody

Detection of Human Semaphorin 4C antibody by Western Blot.

Detection of Human Semaphorin 4C by Western Blot.

Western blot shows lysates of SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6125) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Semaphorin 4C at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Semaphorin 4C antibody in Human Brain by Immunohistochemistry (IHC-P).

Semaphorin 4C in Human Brain.

Semaphorin 4C was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6125) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Semaphorin 4C antibody by Simple WesternTM.

Detection of Human Semaphorin 4C by Simple WesternTM.

Simple Western lane view shows lysates of SW13 human adrenal cortex adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Semaphorin 4C at approximately 121 kDa (as indicated) using 25 µg/mL of Sheep Anti-Human Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6125) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Detection of Semaphorin 4C by Western Blot

Detection of Semaphorin 4C by Western Blot

Signature validation of miRNA-mRNA and proteins found differentially expressed between 402-91/ET and 402-91 cell line. Panel A. qRT-PCR and Western blot analysis showing differences in the expression levels of let-7e and its downstream targets (CCDN1, E2F5, SEMA4C, HMGA1 and HMGA2). Panel B. qRT-PCR and Western blot for miR-21 and its downstream target, PDCD4. qRT-PCR data are the mean of three independent experiments performed in triplicate and calculated with the 2− delta delta Ct method as described in the material and method section. The control 402-91 cells values are arbitrarily set as 1. Bars are +/− SD. * is significant with p<0.05, ** p<0.01, ***p<0.001, as assessed with Student T-test. Western blot is representative of at least two independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22523595), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Semaphorin 4C Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Simple Western

25 µg/mL
Sample: SW13 human adrenal cortex adenocarcinoma cell line

Western Blot

0.5 µg/mL
Sample: SW13 human adrenal cortex adenocarcinoma cell line

Reviewed Applications

Read 1 review rated 3 using AF6125 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 4C

Sema4C (Semaphorin 4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins. It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms. Mature human Sema4C is a type I transmembrane glycoprotein that is 813 amino acids (aa) in length. It contains a 643 aa extracellular region (aa 21-663) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499‑551), and an Ig-like C2-type domain (aa 556-644). The cytoplasmic region interacts with PZD-domain containing proteins. There are three potential splice variants. One demonstrates an alternative start site at Met324, a second shows a deletion of aa 173-211, and a third contains an 89 aa substitution for aa 1-36. Over aa 21‑663, human Sema4C shares 85% aa identity with mouse Sema4C.

Alternate Names

SEMA4C, SEMACL1, Semaf, SEMAI

Entrez Gene IDs

54910 (Human); 20353 (Mouse); 301346 (Rat)

Gene Symbol

SEMA4C

UniProt

Q9C0C4 (Human)

Additional Semaphorin 4C Products

Product Documents for Human Semaphorin 4C Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Semaphorin 4C Antibody

For research use only

Related Research Areas

Proteoglycans

Citations for Human Semaphorin 4C Antibody

Customer Reviews for Human Semaphorin 4C Antibody (1)

3 out of 5
1 Customer Rating
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  • Human Semaphorin 4C Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 07/22/2016

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Protocols

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