Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 209930
View all Serpin A4/Kallistatin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Serpin A4/Kallistatin
Leu22-Pro427
Accession # AAA59454

Specificity

Detects human Serpin A4/Kallistatin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Serpin A1, A3, A5, C1, F2, or recombinant mouse Serpin D1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Serpin A4/Kallistatin Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin A4/Kallistatin (Catalog # 1669-PI), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Serpin A4/Kallistatin (Catalog # 1669-PI)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin A4/Kallistatin

Serpin A4, also called Kallistatin, Kallikrein inhibitor and protease inhibitor 4, is a member of the Serpin superfamily of the serine protease inhibitors (1). Known also as kallistatin or proteinase inhibitor 4, Serpin A4 has been shown to function as a specific inhibitor of tissue kallikrein and as an inhibitor of angiogenesis and tumor growth (2, 3).

References

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Chen, V.C. et al. (2000) J. Biol. Chem. 275:40371.
  3. Miao, R.Q. et al. (2002) Blood 100:3245.

Alternate Names

Kallistatin

Entrez Gene IDs

5267 (Human)

Gene Symbol

SERPINA4

UniProt

AAA59454

Additional Serpin A4/Kallistatin Products

Product Documents for Human Serpin A4/Kallistatin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Serpin A4/Kallistatin Antibody

For research use only

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