Human Serpin A8/AGT DuoSet ELISA

R&D Systems | Catalog # DY3156-05

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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

187-12000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human Serpin A8/AGT DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all Serpin A8/Angiotensinogen ELISA Kits »

Product Summary for Human Serpin A8/AGT DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Serpin A8 / Angiotensinogen. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human Serpin A8/AGT DuoSet ELISA

Human Serpin A8 / Angiotensinogen ELISA Standard Curve

Human Serpin A8 / Angiotensinogen ELISA Standard Curve

Kit Contents for Human Serpin A8/AGT DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)

Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)

Microplates: (Catalog # DY990), or equivalent

Plate Sealers: (Catalog # DY992), or equivalent

*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Serpin A8/Angiotensinogen

The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

Alternate Names

AGT, Angiotensin II, Angiotensinogen, Aogen, PAT

Entrez Gene IDs

183 (Human); 11606 (Mouse); 24179 (Rat)

Gene Symbol

AGT

Additional Serpin A8/Angiotensinogen Products

Product Documents for Human Serpin A8/AGT DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Serpin A8/AGT DuoSet ELISA

For research use only

Citations for Human Serpin A8/AGT DuoSet ELISA

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  • Human Serpin A8/AGT DuoSet ELISA
    Name: Anonymous
    Sample Tested: EDTA Plasma and Urine
    Verified Customer | Posted 10/23/2020
  • Human Serpin A8/AGT DuoSet ELISA
    Name: Anonymous
    Sample Tested: Urine
    Verified Customer | Posted 07/31/2020
    Human Serpin A8/AGT DuoSet ELISA DY3156-05

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