Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot, ELISA Development (Capture), Surface Plasmon Resonance (SPR)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 369439
View all Serpin A8/Angiotensinogen Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Serpin A8/Angiotensinogen
Asp34-Ala485
Accession # P01019

Specificity

Detects human Serpin A8/Angiotensinogen in direct ELISAs and Western blots. Shows no cross-reactivity with recombinant human Serpin A1, -A3, -A4, -A5, -A6, -C1, -D1, -E2, -F1, -F2, -G1, -I1, -I2, -B6, -B8, -B9, or recombinant mouse Serpin A6.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Serpin A8/Angiotensinogen Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Serpin A8/Angiotensinogen

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin A8/Angiotensinogen

Angiotensinogen, a member of the serpin family (Serpin A8), is synthesized in liver and cleaved by renin to produce angiotensin I. This is further processed by angiotensin converting enzyme (ACE) to yield angiotensin II, a key blood pressure regulator. The amino acid sequence of human AGT is 99%, 68%, 64%, and 61% identical to that of chimpanzee, canine, rat, and mouse.

Alternate Names

AGT, Angiotensin II, Angiotensinogen, Aogen, PAT

Entrez Gene IDs

183 (Human); 11606 (Mouse); 24179 (Rat)

Gene Symbol

AGT

UniProt

P01019

Additional Serpin A8/Angiotensinogen Products

Product Documents for Human Serpin A8/Angiotensinogen Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Serpin A8/Angiotensinogen Antibody

For research use only

Citations for Human Serpin A8/Angiotensinogen Antibody

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