Human SF20/MYDGF Antibody

Catalog # Availability Size / Price Qty
MAB1147-100
MAB1147-SP
Human SF20/MYDGF Antibody in Western Blot
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Human SF20/MYDGF Antibody Summary

Species Reactivity
Human
Specificity
Detects human SF20/MYDGF in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 1009420
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SF20/MYDGF
Met1-Leu173
Accession # Q969H8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human SF20/MYDGF by Western Blot. View Larger

Detection of Human SF20/MYDGF by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, WI‑38 human lung fibroblast cell line, human lung tissue, and human liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SF20/MYDGF Monoclonal Antibody (Catalog # MAB1147) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SF20/MYDGF at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry Detection of SF20/MYDGF on U937 Human Cell Line by Flow Cytometry. View Larger

Detection of SF20/MYDGF on U937 Human Cell Line by Flow Cytometry. U937 Human histiocytic lymphoma cell line was stained with Mouse Anti-Human SF20/MYDGF Monoclonal Antibody (Catalog # MAB1147, filled histogram) or Mouse IgG1 isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.    

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SF20/MYDGF

Human SF20 (Stromal cell-derived growth factor SF20, C19orf10, MYDGF1, Myeloid-derived growth factor1 also formerly known as IL25) is a 15 kDa (predicted)  bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction. SF20 stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1 mediated signaling pathway. It is thought to inhibit cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway and is involved in endothelial cell proliferation and angiogenesis. Human SF20 shares 87% aa identity with mouse SF20.              

Long Name
Stromal Cell-derived Growth Factor SF20
Entrez Gene IDs
56005 (Human); 28106 (Mouse)
Alternate Names
C19orf10; chromosome 19 open reading frame 10; EUROIMAGE1875335; IL25; IL27; IL27w; interleukin 27 working designation; Interleukin-25; Ly6elg; MYDGF; myeloid-derived growth factor; R33729_1; SF20; Stromal cell-derived growth factor SF20; UPF0556 protein C19orf10

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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