Human SH2B1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human SH2B1 by Western Blot.
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Product Details
Supplemental Products

Human SH2B1 Antibody Summary

Species Reactivity
Detects human SH2B1 in direct ELISAs and Western blots.
Monoclonal Mouse IgG2A Clone # 700320
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human SH2B1
Accession # Q9NRF2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SH2B1 antibody by Western Blot. View Larger

Detection of Human SH2B1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SH2B1 Monoclonal Antibody (Catalog # MAB6915) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SH2B1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SH2B1

SH2B1 (SH2 adaptor protein B1), also called PSM (Pro-rich, PH and SH2 domain-containing signaling mediator) is an adaptor protein that mediates signaling between receptor tyrosine kinases and JAK signaling pathways. The 756 amino acid (aa) human SH2B1 contains an N-terminal Phe-zipper dimerization domain (aa 26-82), a nuclear localization sequence (aa 224-233), a PH domain (aa 267-376), and a pY-binding SH2 domain (aa 526-614). Within the region used as an immunogen, human SH2B1 shares 92% aa sequence identity with human and mouse SH2B1. Potential isoforms of 683 and 671 diverge after aa 632; a potential 426 aa form also shows an alternate start site at aa 246.

Long Name
SH2B adaptor protein 1
Entrez Gene IDs
25970 (Human); 20399 (Mouse)
Alternate Names
FLJ30542; KIAA1299; Pro-rich, PH and SH2 domain-containing signaling mediator; PSM; SH2 domain-containing protein 1B; SH2 domain-containing putative adapter SH2-B; SH2B adapter protein 1; SH2B adaptor protein 1; SH2-B signaling protein; SH2B1; SH2BDKFZp547G1110

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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