Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 700320
View all SH2B1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SH2B1
Pro317-Leu467
Accession # Q9NRF2

Specificity

Detects human SH2B1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human SH2B1 Antibody

Detection of Human SH2B1 antibody by Western Blot.

Detection of Human SH2B1 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SH2B1 Monoclonal Antibody (Catalog # MAB6915) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SH2B1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human SH2B1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SH2B1

SH2B1 (SH2 adaptor protein B1), also called PSM (Pro-rich, PH and SH2 domain-containing signaling mediator) is an adaptor protein that mediates signaling between receptor tyrosine kinases and JAK signaling pathways. The 756 amino acid (aa) human SH2B1 contains an N-terminal Phe-zipper dimerization domain (aa 26-82), a nuclear localization sequence (aa 224-233), a PH domain (aa 267-376), and a pY-binding SH2 domain (aa 526-614). Within the region used as an immunogen, human SH2B1 shares 92% aa sequence identity with human and mouse SH2B1. Potential isoforms of 683 and 671 diverge after aa 632; a potential 426 aa form also shows an alternate start site at aa 246.

Long Name

SH2B adaptor protein 1

Alternate Names

PSM

Entrez Gene IDs

25970 (Human); 20399 (Mouse)

Gene Symbol

SH2B1

UniProt

Q9NRF2

Additional SH2B1 Products

Product Documents for Human SH2B1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SH2B1 Antibody

For research use only

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