Detection of Human SH2B1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SH2B1 Monoclonal Antibody (Catalog # MAB6915) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SH2B1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
SH2B1 (SH2 adaptor protein B1), also called PSM (Pro-rich, PH and SH2 domain-containing signaling mediator) is an adaptor protein that mediates signaling between receptor tyrosine kinases and JAK signaling pathways. The 756 amino acid (aa) human SH2B1 contains an N-terminal Phe-zipper dimerization domain (aa 26-82), a nuclear localization sequence (aa 224-233), a PH domain (aa 267-376), and a pY-binding SH2 domain (aa 526-614). Within the region used as an immunogen, human SH2B1 shares 92% aa sequence identity with human and mouse SH2B1. Potential isoforms of 683 and 671 diverge after aa 632; a potential 426 aa form also shows an alternate start site at aa 246.
SH2B adaptor protein 1
Entrez Gene IDs:
25970 (Human); 20399 (Mouse)
FLJ30542; KIAA1299; Pro-rich, PH and SH2 domain-containing signaling mediator; PSM; SH2 domain-containing protein 1B; SH2 domain-containing putative adapter SH2-B; SH2B adapter protein 1; SH2B adaptor protein 1; SH2-B signaling protein; SH2BDKFZp547G1110
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