Human SH2B1 Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human SH2B1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SH2B1 Monoclonal Antibody (Catalog # MAB6915) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SH2B1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Background: SH2B1

SH2B1 (SH2 adaptor protein B1), also called PSM (Pro-rich, PH and SH2 domain-containing signaling mediator) is an adaptor protein that mediates signaling between receptor tyrosine kinases and JAK signaling pathways. The 756 amino acid (aa) human SH2B1 contains an N-terminal Phe-zipper dimerization domain (aa 26-82), a nuclear localization sequence (aa 224-233), a PH domain (aa 267-376), and a pY-binding SH2 domain (aa 526-614). Within the region used as an immunogen, human SH2B1 shares 92% aa sequence identity with human and mouse SH2B1. Potential isoforms of 683 and 671 diverge after aa 632; a potential 426 aa form also shows an alternate start site at aa 246.

Product Datasheets

Related Research Areas

• Adaptor Proteins
• Adaptor Proteins in the Akt Pathway
• Positive Regulators of the Jak/STAT Pathway