Human Siglec-16 Antibody Summary
Accession # A6NMB1
Complete Your Research
Complete Your Experiment
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Siglec-16 in HEK293 Human Cell Line Transfected with Human Siglec-16 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with human Siglec-16 and eGFP was stained with (A) Mouse Anti-Human Siglec-16 Monoclonal Antibody (Catalog # MAB68192) or (B) Mouse IgG1 Isotype Control Antibody (MAB002) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).Staining was done using our Staining Membrane-associated Proteins protocol.
Detection of Siglec‑16 in CHO cells transfected with Human Siglec-16 or irrelevant Transfectant cells by Flow Cytometry. CHO cells transfected with human Siglec-16 (filled histogram) or irrelevant transfectant cells (open histogram) were stained with Mouse Anti-Human Siglec-16 Monoclonal Antibody (Catalog # MAB68192), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Siglec-16 (Sialic acid-binding Ig-like lectin 16) is a 58-60 kDa member of the CD33-related SIGLEC family of proteins. It is expressed on macrophages and microglia, and based on Siglec-11, likely binds to an alpha 2,8-linked sialic acid motif. Although Siglec-16 is assumed to have arisen from a Siglec-11 gene duplication and conversion, it is not an inhibitory receptor but an activating one, and possesses a transmembrane (TM)
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human Siglec-16 Antibody
There are currently no reviews for this product. Be the first to review Human Siglec-16 Antibody and earn rewards!
Have you used Human Siglec-16 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image