Human Sirtuin 3/SIRT3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Sirtuin 3/SIRT3 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Sirtuin 2 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 850911
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Sirtuin 3/SIRT3
    Ser101-Lys399
    Accession # Q9NTG7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Sirtuin 3/SIRT3 by Western Blot. Western blot shows lysates of human liver tissue, human heart ventricle tissue, and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Sirtuin 3/SIRT3 Monoclonal Antibody (Catalog # MAB7488) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sirtuin 3/SIRT3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sirtuin 3/SIRT3
Sirtuin 3 (SIRT3; SIR2-like protein 3) is a 28-30 kDa, NAD+-dependent class III member of the sirtuin protein family. It is widely expressed and found apparently in both the nucleus where it deacetylates histones, and in the mitochondrial matrix where it is involved in the regulation of both oxidative stress and the physiology associated with fasting or caloric restriction. Relative to the mitochondria, SIRT3 deacetylates multiple enzyme substrates including SOD2, IDH2, LCAD, OTC and HMGCS2, an action that results in enzyme activation. Human SIRT3 is 399 amino acids (aa) in length. It contains a mitochondrial targeting sequence within the first 100 aa, followed by a sirtuin-type deacetylase domain (aa 126-382) that contains an NAD binding segment (aa 145-165). SIRT3 is initially synthesized as a 42-44 kDa "precursor" in the cytosol. From here, it can translocate into mitochondria where it is cleaved between Arg99Arg100 to generate a 28-30 kDa short form. There is one potential alternative start site at Met143. Over aa 101-399, human and mouse SIRT3 share 84% aa sequence identity.
  • Long Name:
    Silent Mating Type Information Regulation 3 Homolog
  • Entrez Gene IDs:
    23410 (Human); 64384 (Mouse); 293615 (Rat)
  • Alternate Names:
    EC 3.5.1; EC 3.5.1.-; hSIRT3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase; NAD-dependent deacetylase sirtuin-3, mitochondrial; SIR2L3; SIR2L3silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; SIR2-like protein 3; SIRT3; sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae); sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3; Sirtuin 3; sirtuin type 3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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