Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 888418
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Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human SLC14A1
Accession # Q13336

Specificity

Detects human SLC14A1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human SLC14A1 Antibody

Detection of SLC14A1 antibody in Human Red Blood Cells antibody by Flow Cytometry.

Detection of SLC14A1 in Human Red Blood Cells by Flow Cytometry.

Human red blood cells were stained with Mouse Anti-Human SLC14A1 Monoclonal Antibody (Catalog # MAB8238, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Detection of SLC14A1 antibody in HEK293 Human Cell Line Transfected with Human SLC14A1 and eGFP antibody by Flow Cytometry.

Detection of SLC14A1 in HEK293 Human Cell Line Transfected with Human SLC14A1 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human SLC14A1 and eGFP was stained with either (A) Mouse Anti-Human SLC14A1 Monoclonal Antibody (Catalog # MAB8238) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human SLC14A1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human red blood cells and HEK293 human embryonic kidney cell line transfected with human SLC14A1 and eGFP

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLC14A1

Urea transporter 1, designated Solute Carrier family 14 member 1 (SLC14A1) is a 43-54 kDa multipass urea transporter mainly expressed in erythrocytes, but also found in kidney, mesenchymal stem cells, and some carcinomas. It contains an epitope identified as the Kidd (JK) blood group antigen. The antigenic forms differ at only one amino acid (aa); Asp280 represents Jk(a) and Asn280 represents Jk(b). SLC14A1 Isoform 2 (445 aa) contains an extended N-terminus as compared to Isoform 1 (389 aa). Human SLC14A1 shares 83% and 81% aa sequence identity with mouse and rat SLC14A1, respectively.

Long Name

Solute Carrier Family 14 (Urea Transporter), Member 1 (Kidd Blood Group

Alternate Names

HUT11, JK, RACH1, RACH2, UT-B1, UT1, UTE

Entrez Gene IDs

6563 (Human); 108052 (Mouse); 54301 (Rat)

Gene Symbol

SLC14A1

UniProt

Additional SLC14A1 Products

Product Documents for Human SLC14A1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SLC14A1 Antibody

For research use only

Related Research Areas

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