Human SLP-76/LCP2 Antibody

Catalog # Availability Size / Price Qty
MAB5637
MAB5637-SP
Detection of Human SLP‑76/LCP2 by Western Blot.
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Human SLP-76/LCP2 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human SLP-76 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 460004
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SLP-76
Met1-Pro533
Accession # Q13094
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human SLP‑76/LCP2 by Western Blot. View Larger

Detection of Human SLP‑76/LCP2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human SLP‑76/LCP2 Monoclonal Antibody (Catalog # MAB5637) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SLP‑76/LCP2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLP-76/LCP2

SLP-76 is a 533 amino acid (aa), 76 kDa tyrosine phosphorylated intracellular docking protein with a single SH2 recognition domain. It is expressed in T cells, platelets, neutrophils, NK and mast cells, and is required for progression of T cells to double positive stage in the thymus. Tyrosine phosphorylation is required for interaction with the signaling proteins VAV, NCK, PLC gamma, ITK, and ZAP70. SLP-76 may be phosphorylated by ZAP70 leading to NF-AT and IL2 gene activation. Human SLP-76 shares 83% and 85% aa sequence identity with mouse and rat SLP-76, respectively.

Long Name
SH2 Domain-containing Leukocyte Protein of 76 kDa
Entrez Gene IDs
3937 (Human); 16822 (Mouse); 155918 (Rat)
Alternate Names
LCP2; lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of76kDa); lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of76kD); lymphocyte cytosolic protein 2; SH2 domain-containing leukocyte protein of 76 kDa; SH2 domain-containing leukocyte protein of 76kD; SLP-76 tyrosine phosphoprotein; SLP76; SLP-76; SLP7676 kDa tyrosine phosphoprotein

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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