Human SLP-76/LCP2 Antibody (460004)

R&D Systems | Catalog # MAB5637

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 460004
View all SLP-76/LCP2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SLP-76
Met1-Pro533
Accession # Q13094

Specificity

Detects endogenous human SLP-76 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human SLP-76/LCP2 Antibody (460004)

Detection of Human SLP‑76/LCP2 antibody by Western Blot.

Detection of Human SLP‑76/LCP2 by Western Blot.

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human SLP-76/LCP2 Monoclonal Antibody (Catalog # MAB5637) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SLP-76/LCP2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human SLP-76/LCP2 Antibody (460004)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: THP-1 human acute monocytic leukemia cell line and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLP-76/LCP2

SLP-76 is a 533 amino acid (aa), 76 kDa tyrosine phosphorylated intracellular docking protein with a single SH2 recognition domain. It is expressed in T cells, platelets, neutrophils, NK and mast cells, and is required for progression of T cells to double positive stage in the thymus. Tyrosine phosphorylation is required for interaction with the signaling proteins VAV, NCK, PLC gamma, ITK, and ZAP70. SLP-76 may be phosphorylated by ZAP70 leading to NF-AT and IL2 gene activation. Human SLP-76 shares 83% and 85% aa sequence identity with mouse and rat SLP-76, respectively.

Long Name

SH2 Domain-containing Leukocyte Protein of 76 kDa

Alternate Names

LCP2, SLP76

Entrez Gene IDs

3937 (Human); 16822 (Mouse); 155918 (Rat)

Gene Symbol

LCP2

UniProt

Q13094

Additional SLP-76/LCP2 Products

Product Documents for Human SLP-76/LCP2 Antibody (460004)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SLP-76/LCP2 Antibody (460004)

For research use only

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