Human SLP-76/LCP2 Antibody

Catalog #: MAB5637 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5637
MAB5637-SP

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Detection of Human SLP‑76/LCP2 by Western Blot.

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All SLP-76/LCP2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SLP-76/LCP2 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human SLP-76 in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 460004

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SLP-76
Met1-Pro533
Accession # Q13094

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SLP‑76/LCP2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human SLP-76/LCP2 Monoclonal Antibody (Catalog # MAB5637) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SLP-76/LCP2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLP-76/LCP2

SLP-76 is a 533 amino acid (aa), 76 kDa tyrosine phosphorylated intracellular docking protein with a single SH2 recognition domain. It is expressed in T cells, platelets, neutrophils, NK and mast cells, and is required for progression of T cells to double positive stage in the thymus. Tyrosine phosphorylation is required for interaction with the signaling proteins VAV, NCK, PLC gamma, ITK, and ZAP70. SLP-76 may be phosphorylated by ZAP70 leading to NF-AT and IL2 gene activation. Human SLP-76 shares 83% and 85% aa sequence identity with mouse and rat SLP-76, respectively.

Long Name

SH2 Domain-containing Leukocyte Protein of 76 kDa

Entrez Gene IDs

3937 (Human); 16822 (Mouse); 155918 (Rat)

Alternate Names

LCP2; lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of76kDa); lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of76kD); lymphocyte cytosolic protein 2; SH2 domain-containing leukocyte protein of 76 kDa; SH2 domain-containing leukocyte protein of 76kD; SLP-76 tyrosine phosphoprotein; SLP76; SLP-76; SLP7676 kDa tyrosine phosphoprotein