Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 253327
View all Smad4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Smad4
Pro139-Asp332
Accession # Q13485

Specificity

Detects endogenous human Smad4 in Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human Smad1, 5, 6, 7, or 9.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Human Smad4 Antibody

Detection of Human Smad4 antibody by Western Blot.

Detection of Human Smad4 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Human Smad4 Monoclonal Antibody (Catalog # MAB20971) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Smad4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Smad4 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and HEK293 human embryonic kidney cell line

Reviewed Applications

Read 1 review rated 5 using MAB20971 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Smad4

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. Upon signal-induced phosphorylation, Smad subunits associate with the common-mediator subunit, Smad4. This heteromeric complex then translocates into the nucleus to exert transcriptional comodulator activity.

Long Name

Mothers Against DPP Homolog 4

Alternate Names

DPC4, MADH4

Entrez Gene IDs

4089 (Human)

Gene Symbol

SMAD4

UniProt

Q13485

Additional Smad4 Products

Product Documents for Human Smad4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Smad4 Antibody

For research use only

Customer Reviews for Human Smad4 Antibody (1)

5 out of 5
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  • Human Smad4 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 02/07/2018

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