Human SPARC-like 1/SPARCL1 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Human SPARC-like 1 / SPARCL1 ELISA Standard Curve
Preparation and Storage
Background: SPARC-like 1/SPARCL1
SPARC-L1 (Secreted Protein, Acidic and Rich in Cysteines-like 1), also known as Hevin, SC1 or MAST9, is a member of the SPARC family of extracellular glycoproteins. SPARC-L1 is a widely expressed protein with antiadhesive properties. It is thought to terminate neuronal migration by antagonizing adhesion, and promote extravasation in tonsil high endothelial venules by inducing endothelial cell dissociation. SPARC-L1 binds collagen and contributes to formation of dermal collagen fibrils.
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