SPINK1 (Serine Protease Inhibitor Kazal-type 1; also TATI and PST1) is a 6-7 kDa secreted polypeptide initially identified as a tumor-derived trypsin inhibitor. It is widely expressed, and found in cells diverse as pancreatic acinar cells, columnar cells of the stomach, renal collecting duct epithelium, and ureteric transitional plus breast epithelium. SPINK1 is known to be secreted with pancreatic zymogens, and apparently inactivates prematurely-activated trypsin, thus protecting the pancreas from trypsin-mediated enzyme activation. It also is reported to regulate cell migration and proliferation, the latter effect attributed to its structural resemblance to EGF, and its ability to bind to activate the EGFR. Mature human SPINK1 is 56 amino acids (aa) in length (aa 24-79). It contains one Kazal-like domain (aa 26-79) that possesses a potential proteolytic cleavage site between Lys41-Ile42. An 11-12 kDa form in SDS-PAGE has been reported for SPINK1, possibly reflecting dimerization. Mature human SPINK1 shares 66% aa sequence identity with mouse Spink3, the mouse equivalent to human SPINK1.
Human SPINK1 Antibody
R&D Systems | Catalog # MAB74961
Key Product Details
Species Reactivity
Human
Applications
Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 839304
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Product Specifications
Immunogen
E. coli-derived recombinant human SPINK1
Asp24-Cys79
Accession # P00995
Asp24-Cys79
Accession # P00995
Specificity
Detects human SPINK1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human SPINK1 Antibody
Neutralization of SPINK1 Activity by Human SPINK1 Antibody.
Trypsin (12.5 ng/mL) activity is measured in the presence of Recombinant Human SPINK1 (50 ng/mL, 7496-PI) that has been preincubated with increasing concentration of Mouse Anti-Human SPINK1 Monoclonal Antibody (Catalog # MAB74961). The ND50 is approximately 0.8 µg/mL.Applications for Human SPINK1 Antibody
Application
Recommended Usage
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: SPINK1
Long Name
Serine Peptidase Inhibitor, Kazal Type 1
Alternate Names
PCTT, PSTI, Spink3, TATI
Gene Symbol
SPINK1
UniProt
Additional SPINK1 Products
Product Documents for Human SPINK1 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human SPINK1 Antibody
For research use only
Related Research Areas
Citations for Human SPINK1 Antibody
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