Human SPINK1 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Human SPINK1 ELISA Standard Curve
Preparation and Storage
Citations for Human SPINK1 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Androgen deprivation upregulates SPINK1 expression and potentiates cellular plasticity in prostate cancer
Authors: R Tiwari, N Manzar, V Bhatia, A Yadav, MA Nengroo, D Datta, S Carskadon, N Gupta, M Sigouros, F Khani, M Poutanen, A Zoubeidi, H Beltran, N Palanisamy, B Ateeq
Nat Commun, 2020;11(1):384.
Sample Types: Cell Lysates
Targeting SPINK1 in the damaged tumour microenvironment alleviates therapeutic resistance
Authors: F Chen, Q Long, D Fu, D Zhu, Y Ji, L Han, B Zhang, Q Xu, B Liu, Y Li, S Wu, C Yang, M Qian, J Xu, S Liu, L Cao, YE Chin, EW Lam, JP Coppé, Y Sun
Nat Commun, 2018;9(1):4315.
Sample Types: Serum
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