Human SPINK1 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Human SPINK1 ELISA Standard Curve
Preparation and Storage
Citations for Human SPINK1 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
Filter your results:
SPINK1 as a plasma marker for tumor hypoxia and a therapeutic target for radiosensitization
Authors: T Suwa, M Kobayashi, Y Shirai, JM Nam, Y Tabuchi, N Takeda, S Akamatsu, O Ogawa, T Mizowaki, EM Hammond, H Harada
JCI Insight, 2021;6(21):.
Sample Types: Plasma
Androgen deprivation upregulates SPINK1 expression and potentiates cellular plasticity in prostate cancer
Authors: R Tiwari, N Manzar, V Bhatia, A Yadav, MA Nengroo, D Datta, S Carskadon, N Gupta, M Sigouros, F Khani, M Poutanen, A Zoubeidi, H Beltran, N Palanisamy, B Ateeq
Nat Commun, 2020;11(1):384.
Sample Types: Cell Lysates
Plasma biomarkers of the amyloid pathway are associated with geographic atrophy secondary to age-related macular degeneration
Authors: K Lashkari, GC Teague, U Beattie, J Betts, S Kumar, MM McLaughlin, FJ López
PLoS ONE, 2020;15(8):e0236283.
Sample Types: Plasma
Targeting SPINK1 in the damaged tumour microenvironment alleviates therapeutic resistance
Authors: F Chen, Q Long, D Fu, D Zhu, Y Ji, L Han, B Zhang, Q Xu, B Liu, Y Li, S Wu, C Yang, M Qian, J Xu, S Liu, L Cao, YE Chin, EW Lam, JP Coppé, Y Sun
Nat Commun, 2018;9(1):4315.
Sample Types: Serum
No product specific FAQs exist for this product, however you mayView all ELISA FAQs
DuoSet Ancillary Reagent Kits
Supplemental ELISA Products
Reviews for Human SPINK1 DuoSet ELISA
There are currently no reviews for this product. Be the first to review Human SPINK1 DuoSet ELISA and earn rewards!
Have you used Human SPINK1 DuoSet ELISA?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image