Detection of Human ST6 Gal Sialyltransferase 2/ST6GAL2 by Western Blot.
Western blot shows lysates of human brain (motor cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ST6 Gal Sialyltransferase 2/ST6GAL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7747) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST6 Gal Sialyltransferase 2/ST6GAL2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ST6 Gal Sialyltransferase 2/ST6GAL2 in Human Brain.
ST6 Gal Sialyltransferase 2/ST6GAL2 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human ST6 Gal Sialyltransferase 2/ST6GAL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7747) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: ST6 Gal Sialyltransferase 2/ST6GAL2
ST6GAL2 (Sialyltransferase 2; also beta -galactoside alpha -2,6 Sialyltransferase 2) is a 60-62 kDa member of the glycosyltransferase 29 family of enzymes. It has restricted expression, likely being found in neurons and bronchial eipthelium. ST6GAL2 transfers sialic acid from CMP-Neu5Ac to a galactose that is linked to N-Aceylglucosamine through a beta 1-4 linkage (Gal beta 1-4GlcNac). This transfer will occur when the target motif is found either on select glycoproteins or free oligosaccharides. Notably, the kinetics of this enzyme suggest additional biological functions other than sialic acid transfer. Human ST6GAL2 is a 529 amino acid (aa) type II transmembrane Golgi protein. It contains an 11 aa N-terminal cytoplasmic region and a 497 aa luminal domain (aa 33-529). The luminal domain possesses an L motif that binds CMP-Sialic acid (aa 296-343), an S motif that binds CMP-Sialic acid and galactose (aa 444-458) and a VS motif that is involved with the enzyme's catalytic activity (aa 483-488). There is some evidence that ST6GAL2 may form homodimers. There is one splice variant that shows a 26 aa substitution for aa 441-529. Over aa 243-529, human ST6GAL2 shares 88% aa sequence identity with mouse ST6GAL2.
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