Human ST6 Gal Sialyltransferase 2/ST6GAL2 Antibody

Catalog # Availability Size / Price Qty
AF7747
AF7747-SP
Detection of Human ST6 Gal Sialyltransferase 2/ST6GAL2 by Western Blot.
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Citations (2)
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Human ST6 Gal Sialyltransferase 2/ST6GAL2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ST6 Gal Sialyltransferase 2/ST6GAL2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human ST6GAL1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ST6 Gal Sialyltransferase 2/ST6GAL2
Glu243-Ser529
Accession # Q96JF0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human ST6 Gal Sialyltransferase 2/ST6GAL2 by Western Blot. View Larger

Detection of Human ST6 Gal Sialyltransferase 2/ST6GAL2 by Western Blot. Western blot shows lysates of human brain (motor cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ST6 Gal Sialyltransferase 2/ST6GAL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7747) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST6 Gal Sialyltransferase 2/ST6GAL2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry ST6 Gal Sialyltransferase 2/ST6GAL2 in Human Brain. View Larger

ST6 Gal Sialyltransferase 2/ST6GAL2 in Human Brain. ST6 Gal Sialyltransferase 2/ST6GAL2 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human ST6 Gal Sialyltransferase 2/ST6GAL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7747) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter­stained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ST6 Gal Sialyltransferase 2/ST6GAL2

ST6GAL2 (Sialyltransferase 2; also beta -galactoside alpha -2,6 Sialyltransferase 2) is a 60-62 kDa member of the glycosyltransferase 29 family of enzymes. It has restricted expression, likely being found in neurons and bronchial eipthelium. ST6GAL2 transfers sialic acid from CMP-Neu5Ac to a galactose that is linked to N‑Aceylglucosamine through a beta 1-4 linkage (Gal beta 1-4GlcNac). This transfer will occur when the target motif is found either on select glycoproteins or free oligosaccharides. Notably, the kinetics of this enzyme suggest additional biological functions other than sialic acid transfer. Human ST6GAL2 is a 529 amino acid (aa) type II transmembrane Golgi protein. It contains an 11 aa N‑terminal cytoplasmic region and a 497 aa luminal domain (aa 33-529). The luminal domain possesses an L motif that binds CMP-Sialic acid (aa 296-343), an S motif that binds CMP-Sialic acid and galactose (aa 444-458) and a VS motif that is involved with the enzyme's catalytic activity (aa 483-488). There is some evidence that ST6GAL2 may form homodimers. There is one splice variant that shows a 26 aa substitution for aa 441‑529. Over aa 243-529, human ST6GAL2 shares 88% aa sequence identity with mouse ST6GAL2.

Long Name
ST6 beta-Galactoside alpha-2,6-Sialyltransferase 2
Entrez Gene IDs
84620 (Human); 240119 (Mouse); 301155 (Rat)
Alternate Names
Alpha 2,6-ST 2; beta-galactoside alpha-2,6-sialyltransferase 2; beta-galactoside alpha-2,6-sialyltransferase II; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 2; EC 2.4.99; EC 2.4.99.1; FLJ30711; FLJ37730; FLJ38334; hST6Gal II; KIAA1877; KIAA1877St6gal2; sialyltransferase 2 (monosialoganglioside sialyltransferase); Sialyltransferase 2; SIAT2; ST6 beta-galactosamide alpha-2,6-sialyltranferase 2; ST6Gal II; ST6GAL2; St6GalII

Product Datasheets

Citations for Human ST6 Gal Sialyltransferase 2/ST6GAL2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Sialidase inhibitors attenuate pulmonary fibrosis in a mouse model
    Authors: TR Karhadkar, D Pilling, N Cox, RH Gomer
    Sci Rep, 2017;7(1):15069.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Identification of target proteins involved in cochlear otosclerosis.
    Authors: Richard C, Doherty J, Fayad J, Cordero A, Linthicum F
    Otol Neurotol, 0;36(5):923-31.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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