Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1076415
View all TCEB1 Primary Antibodies »

Product Specifications

Immunogen

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VHL/ELOB/ELOC/CUL2/RBX1 Complex
Accession # Q15369

Specificity

Detects hElongin B/Elongin C/VHL/CUL2/RBX1 Complex in Direct ELISA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TCEB1 Antibody

Detection of TCEB1 in Human Liver.

TCEB1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human TCEB1 Monoclonal Antibody (Catalog # MAB11569) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of TCEB1 in MCF‑7 Human Cell Line.

TCEB1 was detected in fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human TCEB1 Monoclonal Antibody (Catalog # MAB11569) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human TCEB1 Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: fixed MCF-7 human breast cancer cell line

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TCEB1

Elongin C is a 12kDa, 112aa general transcription elongation factor.  Elongin C, along with Elongin B, Cul2 and Rbx1 to forms a complex with VHL, and Elongin C is required for the function of VHL.  Mutations in Elongin C are responsible for 0.5% to 5% of clear cell renal cell carcinomas.  Tumorigenic mutations frequently occur in a 35-residume domain of VHL responsible for Elongin C binding.

References

  1. Brugarolas J. Molecular genetics of clear-cell renal cell carcinoma. J Clin Oncol. 2014 Jun 20;32(18):1968-76. doi: 10.1200/JCO.2012.45.2003. Epub 2014 May 12. PMID: 24821879; PMCID: PMC4050206.   
  2. Stebbins CE, Kaelin WG Jr, Pavletich NP. Structure of the VHL-ElonginC-ElonginB complex: implications for VHL tumor suppressor function. Science. 1999 Apr 16;284(5413):455-61. doi: 10.1126/science.284.5413.455. PMID: 10205047.   
  3. 3.Hakimi AA, Tickoo SK, Jacobsen A, Sarungbam J, Sfakianos JP, Sato Y, Morikawa T, Kume H, Fukayama M, Homma Y, Chen YB, Sankin A, Mano R, Coleman JA, Russo P, Ogawa S, Sander C, Hsieh JJ, Reuter VE. TCEB1-mutated renal cell carcinoma: a distinct genomic and morphological subtype. Mod Pathol. 2015 Jun;28(6):845-853. doi: 10.1038/modpathol.2015.6. Epub 2015 Feb 13. PMID: 25676555; PMCID: PMC4449825.

Long Name

TCEB1

Alternate Names

ELOC, elongin 15 kDa subunit, elongin-C, RNA polymerase II transcription factor SIII subuni, SIII, SIII p15, transcription elongation factor B (SIII), polypept, transcription elongation factor B polypeptide 1, transcription elongation factor B subunit 1

Entrez Gene IDs

6921 (Human); 67923 (Mouse); 64525 (Rat)

Gene Symbol

Q15369

UniProt

Q15369 (Human)

Additional TCEB1 Products

Product Documents for Human TCEB1 Antibody

Certificate of Analysis

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Product Specific Notices for Human TCEB1 Antibody

For research use only

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Protocols

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