Human Testican 1/SPOCK1 Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (2)
Supplemental Products

Human Testican 1/SPOCK1 Antibody Summary

Species Reactivity
Detects human Testican 1/SPOCK1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Testican 2 or rhTestican 3 is observed.
Monoclonal Mouse IgG1 Clone # 316801
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Testican 1/SPOCK1
Accession # Q08629
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human Testican 1/SPOCK1 (Catalog # 2327-PI)
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Testican 1/SPOCK1 (Catalog # 2327-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Testican 1/SPOCK1

Testican 1, also known as SPOCK1, was originally identified in seminal plasma and is also expressed in brain. It is a proteoglycan that contains a Ca2+-binding domain and a C-terminal acidic domain with putative glycosaminoglycan attachment sites as well as three potential inhibitory domains targeted toward three different classes of proteases, metallo-, cysteine and serine proteases. Human Testican 1 shares 95% amino acid sequence identity with mouse Testican 1.

Entrez Gene IDs
6695 (Human)
Alternate Names
FLJ37170; Protein SPOCK; sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1; SPOCK1; SPOCKcwcv and kazal-like domains proteoglycan (testican); Testican 1; TIC1; TICN1

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Citations for Human Testican 1/SPOCK1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. SPOCK1 is a novel inducer of epithelial to mesenchymal transition in drug-induced gingival overgrowth
    Authors: R Alshargabi, T Sano, A Yamashita, A Takano, T Sanada, M Iwashita, T Shinjo, T Fukuda, T Sanui, S Kishida, F Nishimura
    Sci Rep, 2020;10(1):9785.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Broad regulation of matrix and adhesion molecules in THP-1 human macrophages by nitroglycerin.
    Authors: Krishnatry AS, Brazeau DA, Fung HL
    Nitric Oxide, 2010;22(1):11-7.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot


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