Human TFF2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human TFF2 by Western Blot.
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Product Details
Supplemental Products

Human TFF2 Antibody Summary

Species Reactivity
Detects human TFF2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human TFF3 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human TFF2
Accession # Q03403
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TFF2 antibody by Western Blot. View Larger

Detection of Human TFF2 by Western Blot. Western blot shows lysates of human stomach tissue, human stomach cancer tissue, human breast tissue, and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TFF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4077) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TFF2 at approximately 19-21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFF2

Trefoil factor 2 (TFF2), also known as spasmolytic polypeptide (Sp) is a 20 kDa secreted trefoil factor family protein. It is a protease-resistant glycoprotein primarily produced by mucous-secreting cells of the stomach and duodenum and is upregulated in response to inflammation. TFF2 interacts with mucin and other TFF2-binding proteins to protect and repair mucosal epithelium. TFF2 is also expressed by macrophages and lymphocytes and may have a role in modulating immune system responses. The 106 residue mature human TFF2 contains two trefoil domains (aa 36-72 and aa 80-121) and one N-linked glycosylation site. Human TFF2 shares 71% and 87% aa sequence identity with canine and mouse TFF2, respectively.

Long Name
Trefoil Factor 2
Entrez Gene IDs
7032 (Human); 21785 (Mouse); 116592 (Rat)
Alternate Names
SML1; SML1trefoil factor 2, SML1, human spasmolytic polypeptide (SP)10spasmolytic protein 1; SP; Spasmolysin; Spasmolytic Polypeptide; TFF2; trefoil factor 2

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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