Human TNF RII/TNFRSF1B Antibody Summary
Applications
under non-reducing conditions only
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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TNF RII/TNFRSF1B in Human Peripheral Blood Lymphocytes. TNF RII/TNFRSF1B was detected in immersion fixed human peripheral blood lymphocytes using 5 µg/mL Goat Anti-Human TNF RII/TNFRSF1B Polyclonal Antibody (Catalog # AB‑226‑PB) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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TNF RII/TNFRSF1B Specificity is Shown by Immunocytochemistry in Knockout Cell Line. TNF RII/TNFRSF1B was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in TNF RII/TNFRSF1B knockout (KO) THP‑1 Human cell line using Goat Anti-Human TNF RII/TNFRSF1B Polyclonal Antibody (Catalog # AB-226-PB) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF RII/TNFRSF1B
TNF RII is a type I transmembrane glycoprotein primarily expressed on hematopoietic cells and is designated TNFRSF1B. TNF RII is a highaffinity receptor for TNF alpha and TNF beta. A soluble form of the receptor generated by proteolytic cleavage functions as a TNF antagonist.
Product Datasheets
Citations for Human TNF RII/TNFRSF1B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
Dig. Dis. Sci., 2007-03-30;52(10):2775-83.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
B cell maturation antigen, the receptor for a proliferation-inducing ligand and B cell-activating factor of the TNF family, induces antigen presentation in B cells.
Authors: Yang M, Hase H, Legarda-Addison D, Varughese L, Seed B, Ting AT
J. Immunol., 2005-09-01;175(5):2814-24.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
trans fatty acids and systemic inflammation in heart failure.
Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development
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