Human TNF RII/TNFRSF1B Antibody

Name: Human TNF RII/TNFRSF1B Antibody
Brand: R&D Systems
SKU: AB-226-PB

R&D Systems | Catalog # AB-226-PB

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Citations (3)

Product Documents

Discontinued Product

AB-226-PB has been discontinued. An alternative/replacement product is available: AF726, MAB726. View all TNF RII/TNFRSF1B products.

Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, Immunocytochemistry

Cited:

Flow Cytometry, Immunocytochemistry, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all TNF RII/TNFRSF1B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human sTNF RII

Specificity

Detects human TNF RII in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TNF RII/TNFRSF1B Antibody

TNF RII/TNFRSF1B in Human Peripheral Blood Lymphocytes.

TNF RII/TNFRSF1B was detected in immersion fixed human peripheral blood lymphocytes using 5 µg/mL Goat Anti-Human TNF RII/TNFRSF1B Polyclonal Antibody (Catalog # AB‑226‑PB) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

TNF RII/TNFRSF1B Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

TNF RII/TNFRSF1B was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in TNF RII/TNFRSF1B knockout (KO) THP‑1 Human cell line using Goat Anti-Human TNF RII/TNFRSF1B Polyclonal Antibody (Catalog # AB-226-PB) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human TNF RII/TNFRSF1B Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood lymphocytes

Knockout Validated

TNF RII/TNFRSF1B was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in TNF RII/TNFRSF1B knockout (KO) THP‑1 Human cell line

Western Blot

1 µg/mL
Sample: Recombinant Human TNF RII/TNFRSF1B Fc Chimera (Catalog # 726-R2)
under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TNF RII/TNFRSF1B

TNF RII is a type I transmembrane glycoprotein primarily expressed on hematopoietic cells and is designated TNFRSF1B. TNF RII is a highaffinity receptor for TNF alpha and TNF beta. A soluble form of the receptor generated by proteolytic cleavage functions as a TNF antagonist.