Detects human TNF RII/TNFRSF1B in direct ELISAs and Western blots. In direct ELISAs, less than 50% cross‑reactivity with recombinant mouse TNF RII is observed and less than 5% cross-reactivity with recombinant rat TNF RII is observed.
Polyclonal Goat IgG
E. coli-derived recombinant human TNF RII/TNFRSF1B
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human TNF RII/TNFRSF1B
2.5 µg/106 cells
Human peripheral blood granulocytes
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Measured by its ability to neutralize TNF RII/TNFRSF1B-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.04-0.2 µg/mL in the presence of 0.075 µg/mL Recombinant Human TNF RII/TNFRSF1B Fc Chimera, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
TNF RII/TNFRSF1B Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RII/TNFRSF1B Antibody. Recombinant Human TNF RII/TNFRSF1B Fc Chimera (Catalog # 726‑R2) inhibits Recombinant Human TNF‑ alpha (Catalog # 210‑TA) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human TNF‑ alpha (0.25 ng/mL) activity elicited by Recombinant Human TNF RII/TNFRSF1B Fc Chimera (0.075 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF726). The ND50 is typically 0.04-0.2 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF RII/TNFRSF1B
TNF RII, also known as p75 or p80 TNF R, is a prototypic member of the
TNF receptor superfamily and has been designated TNFRSF1B. It binds TNF
with high affinity and is expressed primarily in cells of the immune
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