Detects human TNF RII in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) TNF RI, recombinant mouse (rm) TNF RI, rmTNF RII, rmTNF‑ alpha, rhTNF‑ alpha, or rhTNF‑ beta. In Western blots, this antibody does not cross‑react with rhTNF RI, rmTNF RI, or rmTNF RII.
Monoclonal Mouse IgG1 Clone # 22210
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human TNF RII/TNFRSF1B Leu23-Asp257 Accession # P20333
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human TNF RII/TNFRSF1B Fc Chimera (Catalog # 726-R2) under non-reducing conditions only
Human TNF RII/TNFRSF1B Sandwich Immunoassay
ELISA Capture (Matched Antibody Pair)
Human TNF RII/TNFRSF1B Antibody (Catalog # MAB726)
ELISA Detection (Matched Antibody Pair)
Human TNF RII/TNFRSF1B Biotinylated Antibody (Catalog # BAF726)
Recombinant Human TNF RII/TNFRSF1B (aa 24-206) Protein (Catalog # 1089-R2)
Measured by its ability to neutralize TNF RII/TNFRSF1B-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 0.3 µg/mL Recombinant Human TNF RII/TNFRSF1B Fc Chimera, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
TNF RII/TNFRSF1B Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RII/TNFRSF1B Antibody. Recombinant Human TNF RII/TNFRSF1B Fc Chimera (Catalog # 726-R2) inhibits Recombinant Human TNF‑ alpha (Catalog # 210-TA) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of Recombinant Human TNF‑ alpha (0.25 ng/mL) activity elicited by Recombinant Human TNF RII/TNFRSF1B Fc Chimera (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB726). The ND50 is typically 0.5-1.5 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF RII/TNFRSF1B
TNF RII, also known as p75 or p80 TNF R, is a prototypic member of the
TNF receptor superfamily and has been designated TNFRSF1B. It binds TNF
with high affinity and is expressed primarily in cells of the immune
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but also provides information about sample types, species, and experimental conditions.
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species: Human.We have 1 review tested in 1 application: ELISA.