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Human TNF RII/TNFRSF1B Antibody
R&D Systems | Catalog # MAB726
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Neutralization, Flow Cytometry, Array Development, Cell Culture, ELISA Capture, ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 22210
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Product Specifications
Immunogen
E. coli-derived recombinant human TNF RII/TNFRSF1B
Leu23-Asp257
Accession # P20333
Leu23-Asp257
Accession # P20333
Specificity
Detects human TNF RII in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) TNF RI, recombinant mouse (rm) TNF RI, rmTNF RII, rmTNF‑ alpha, rhTNF‑ alpha, or rhTNF‑ beta. In Western blots, this antibody does notcross‑react with rhTNF RI, rmTNF RI, or rmTNF RII.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human TNF RII/TNFRSF1B Antibody
TNF RII/TNFRSF1B Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RII/TNFRSF1B Antibody.
Recombinant Human TNF RII/TNFRSF1B Fc Chimera (726-R2) inhibits Recombinant Human TNF-a (210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of Recombinant Human TNF-a (0.25 ng/mL) activity elicited by Recombinant Human TNF RII/TNFRSF1B Fc Chimera (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB726). The ND50 is typically 30.0-300 ng/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).Applications for Human TNF RII/TNFRSF1B Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human TNF RII/TNFRSF1B Fc Chimera (Catalog # 726-R2)
under non-reducing conditions only
Sample: Recombinant Human TNF RII/TNFRSF1B Fc Chimera (Catalog # 726-R2)
under non-reducing conditions only
Neutralization
Measured by its ability to neutralize TNF RII/TNFRSF1B-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 30.0-300 ng/mL in the presence of 0.3 µg/mL Recombinant Human TNF RII/TNFRSF1B Fc Chimera, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.
Human TNF RII/TNFRSF1B Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 5 using MAB726 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TNF RII/TNFRSF1B
Long Name
Tumor Necrosis Factor Receptor II
Alternate Names
CD120b, TNFRII, TNFRSF1B
Gene Symbol
TNFRSF1B
UniProt
Additional TNF RII/TNFRSF1B Products
Product Documents for Human TNF RII/TNFRSF1B Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human TNF RII/TNFRSF1B Antibody
For research use only
Related Research Areas
Citations for Human TNF RII/TNFRSF1B Antibody
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Customer Reviews for Human TNF RII/TNFRSF1B Antibody (4)
5 out of 5
4 Customer Ratings
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Application: ELISASample Tested: SerumSpecies: PrimateVerified Customer | Posted 11/28/2022Used as a capture antibody in our NHP ELISA
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Application: Western BlotSample Tested: SerumSpecies: HumanVerified Customer | Posted 08/28/2021
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Application: Block/NeutralizeSample Tested: Cell culture supernatantSpecies: HumanVerified Customer | Posted 12/15/2017
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Application: ELISASample Tested: Cell culture supernatant and Serum and PlasmaSpecies: HumanVerified Customer | Posted 11/22/2017We used MAB726 as the solid phase antibody, 1089-R2 as the immunoassay standard and BAF225 as the detection antibody in a sandwich ELISA. Everything worked very well.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
IL-15 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
mTOR Signaling Pathway
TNF Superfamily Pathway: Human Ligand-Receptor Interactions & their Associated Functions
