Human Total Bax-alpha DuoSet IC ELISA
Human Total Bax-alpha DuoSet IC ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
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Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15- (96-well) plate pack sizes
- Economical alternative to Western blot
Kit Content
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
- Streptavidin-HRP
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: Bax-alpha
B cell leukemia/lymphoma-2 associated X protein (Bax) is a pro-apoptotic member of the Bcl-2 family of proteins found in many cell types. Bax is a cytosolic protein that translocates to the mitochondria and participates in Cytochrome c release in response to apoptotic stimuli. Aberrant expression of Bcl-2 family members can inappropriately promote or prevent apoptosis. Increased expression of Bax is associated with the apoptotic loss of neurons in Parkinson's disease.
Citations for Human Total Bax-alpha DuoSet IC ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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Pancreatic secretory trypsin inhibitor reduces multi-organ injury caused by gut ischemia/reperfusion in mice
Authors: RJ Playford, T Marchbank
PLoS ONE, 2020;15(1):e0227059.
Species: Human
Sample Types: Cell Lysates
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The Effects of Silver Nanoparticles Biosynthesized Using Fig and Olive Extracts on Cutaneous leishmaniasis Induced Inflammation in Female Balb/c Mice
Authors: MA Almayouf, MF El-Khadrag, MA Awad, EM Al-Olayan
Bioscience Reports, 2020;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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MiR-507 inhibits the growth and invasion of trophoblasts by targeting CAMK4
Authors: HQ Li, JJ Fan, XH Li, D Bao
Eur Rev Med Pharmacol Sci, 2020;24(11):5856-5862.
Species: Human
Sample Types: Cell Lysates
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Probiotics and yogurt modulate oxidative stress and fibrosis in livers of Schistosoma mansoni-infected mice
Authors: MF El-Khadrag, EM Al-Olayan, MIY Elmallah, AM Alharbi, HM Yehia, AE Abdel Mone
BMC Complement Altern Med, 2019;19(1):3.
Species: Mouse
Sample Types: Tissue Homogenates
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Redox correlation in muscle lengthening and immune response in eccentric exercise
Authors: F He, CC Chuang, T Zhou, Q Jiang, DA Sedlock, L Zuo
PLoS ONE, 2018;13(12):e0208799.
Species: Human
Sample Types: Cell Lysates
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Oxidative stress-mediated apoptosis induced by ethanolic mango seed extract in cultured estrogen receptor positive breast cancer MCF-7 cells.
Authors: Abdullah A, Mohammed A, Rasedee A, Mirghani M
Int J Mol Sci, 2015;16(2):3528-36.
Species: Human
Sample Types: Cell Lysates
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Bone morphogenetic proteins and receptors are over-expressed in bone-marrow cells of multiple myeloma patients and support myeloma cells by inducing ID genes.
Authors: Grcevic D, Kusec R, Kovacic N, Lukic A, Lukic IK, Ivcevic S, Nemet D, Seiwerth RS, Ostojic SK, Croucher PI, Marusic A
Leuk. Res., 2010;34(6):742-51.
Species: Human
Sample Types: Cell Lysates
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Engagement of CD31 delivers an activating signal that contributes to the survival of chronic lymphocytic leukaemia cells.
Authors: Poggi A, Prevosto C, Catellani S, Rocco I, Garuti A, Zocchi MR
Br. J. Haematol., 2010;151(3):252-64.
Species: Human
Sample Types: Cell Lysates
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AMP-activated protein kinase-dependent and -independent mechanisms underlying in vitro antiglioma action of compound C.
Authors: Vucicevic L, Misirkic M, Janjetovic K, Harhaji-Trajkovic L, Prica M, Stevanovic D, Isenovic E, Sudar E, Sumarac-Dumanovic M, Micic D, Trajkovic V
Biochem. Pharmacol., 2009;77(11):1684-93.
Species: Human
Sample Types: Cell Lysates
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