Human Total Bax-alpha DuoSet IC ELISA

R&D Systems | Catalog # DYC820-2

R&D Systems
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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

312-20000 pg/mL

Sample Type

Cell Lysates

Reactivity

Human

Human Total Bax-alpha DuoSet IC ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot
View all Bax-alpha ELISA Kits »

Product Summary for Human Total Bax-alpha DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total Bax-alpha in cell lysates. An immobilized capture antibody specific for Bax-alpha binds both phosphorylated and unphosphorylated Bax-alpha. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

Cell lysates (100 µL)

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human Total Bax-alpha DuoSet IC ELISA

Standard Curve

Kit Contents for Human Total Bax-alpha DuoSet IC ELISA

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Bax-alpha

B cell leukemia/lymphoma-2 associated X protein (Bax) is a pro-apoptotic member of the Bcl-2 family of proteins found in many cell types. Bax is a cytosolic protein that translocates to the mitochondria and participates in Cytochrome c release in response to apoptotic stimuli. Aberrant expression of Bcl-2 family members can inappropriately promote or prevent apoptosis. Increased expression of Bax is associated with the apoptotic loss of neurons in Parkinson's disease.

Long Name

Bcl Associated X Protein alpha

Alternate Names

Baxalpha

Entrez Gene IDs

581 (Human); 12028 (Mouse); 24887 (Rat)

Gene Symbol

BAX

Additional Bax-alpha Products

Product Documents for Human Total Bax-alpha DuoSet IC ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Total Bax-alpha DuoSet IC ELISA

For research use only

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Human Total Bax-alpha DuoSet IC ELISA

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