Human Total HO-1/HMOX1 DuoSet IC ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15- (96-well) plate pack sizes
- Economical alternative to Western blot
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: TMB ELISA Substrate (Catalog # DY999B)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Preparation and Storage
Heme Oxygenase (HO), also known as HMOX, is the rate limiting enzyme in the breakdown of heme into Biliverdin, CO, and iron. There are two major isoforms of this enzyme, HO-1 and HO-2. Both have similar enzymatic activity; however, HO-1 is rapidly inducible, while HO-2 is constitutively expressed. Inducers of HO-1 include cell stressors such as oxidative stress, infection, hypoxia, and cytotoxic agents. The reaction products generated by HO-1 activity are biologically active, and the enzyme has a broad range of putative roles. For instance, Biliverdin is an antioxidant with cytoprotective and anti-inflammatory properties, and CO has the potential to suppress inflammation and apoptosis. Because of these activities, HO-1 has received much attention as a therapeutic target for a range of pathological processes.
Citations for Human Total HO-1/HMOX1 DuoSet IC ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 8
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Hydroxyurea treatment is associated with reduced degree of oxidative perturbation in children and adolescents withï¿½sickle cell anemia
Authors: CL Vinhaes, RS Teixeira, JAS Monteiro-J, R Tibúrcio, JM Cubillos-A, MB Arriaga, AG Sabarin, AJ de Souza, JJ Silva, IM Lyra, AM Ladeia, BB Andrade
Sci Rep, 2020;10(1):18982.
Sample Types: Plasma
Heightened Plasma Levels of Transforming Growth Factor Beta (TGF-&beta) and Increased Degree of Systemic Biochemical Perturbation Characterizes Hepatic Steatosis in Overweight Pediatric Patients: A Cross-Sectional Study
Authors: JR Barretto, N Boa-Sorte, CL Vinhaes, H Malta-Sant, J Rebouças-S, CF Ramos, MAS Torres-Nas, VM Borges, BB Andrade
Sample Types: Plasma
Mangiferin Alleviates Ovalbumin-Induced Allergic Rhinitis via Nrf2/HO-1/NF-kappaB Signaling Pathways
Authors: CH Piao, YJ Fan, TV Nguyen, CH Song, OH Chai
Int J Mol Sci, 2020;21(10):.
Sample Types: Nasal Lavage Fluid
Baicalin Exerts Anti-Airway Inflammation and Anti-Remodelling Effects in Severe Stage Rat Model of Chronic Obstructive Pulmonary Disease
Authors: G Wang, N Mohammadtu, Y Lv, H Zhang, J Sun, J Dong
Evid Based Complement Alternat Med, 2018;2018(0):7591348.
Sample Types: Serum
IN VITRO STUDIES ON HEME OXYGENASE-1 AND P24 ANTIGEN HIV-1 LEVEL AFTERHYPERBARIC OXYGEN TREATMENTOFHIV-1 INFECTED ON PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMCS)
Authors: R Budiarti, Kuntaman, Nasronudin, Suryokusum, SQ Khairunisa
Afr J Infect Dis, 2018;12(1):1-6.
Sample Types: Cell Culture Supernates
Comparing the effects of mitochondrial targeted and localized antioxidants with cellular antioxidants in human skin cells exposed to UVA and hydrogen peroxide.
Authors: Oyewole, Anne O, Wilmot, Marie-Cl, Fowler, Mark, Birch-Machin, Mark A
FASEB J, 2014;28(1):485-94.
Sample Types: Whole Cells
Determination of an angiotensin II-regulated proteome in primary human kidney cells by stable isotope labeling of amino acids in cell culture (SILAC).
Authors: Konvalinka, Ana, Zhou, Joyce, Dimitromanolakis, Apostolo, Drabovich, Andrei P, Fang, Fei, Gurley, Susan, Coffman, Thomas, John, Rohan, Zhang, Shao-Lin, Diamandis, Elefther, Scholey, James W
J Biol Chem, 2013;288(34):24834-47.
Sample Types: Cell Lysates
Increased levels of plasma haemoxygenase-1 in prostate cancer.
Authors: Blann AD, Balakrishnan B, Ryan P
Prostate Cancer Prostatic Dis., 2011;14(2):114-7.
Sample Types: Plasma
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