Human Total HO-1/HMOX1 DuoSet IC ELISA

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Product Details
Citations (11)
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Human Total HO-1/HMOX1 DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Assay Length
4 hours 40 mins (after plate preparation)
Sample Volume Required
Cell lysates (100 µL)
Assay Range
156.0 - 10,000 pg/mL
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total HO-1 / HMOX1 / HSP32 in cell lysates. An immobilized capture antibody specific for HO-1 / HMOX1 / HSP32 binds both phosphorylated and unphosphorylated HO-1 / HMOX1 / HSP32. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data

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Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HO-1/HMOX1/HSP32

Heme Oxygenase (HO), also known as HMOX, is the rate limiting enzyme in the breakdown of heme into Biliverdin, CO, and iron. There are two major isoforms of this enzyme, HO-1 and HO-2. Both have similar enzymatic activity; however, HO-1 is rapidly inducible, while HO-2 is constitutively expressed. Inducers of HO-1 include cell stressors such as oxidative stress, infection, hypoxia, and cytotoxic agents. The reaction products generated by HO-1 activity are biologically active, and the enzyme has a broad range of putative roles. For instance, Biliverdin is an antioxidant with cytoprotective and anti-inflammatory properties, and CO has the potential to suppress inflammation and apoptosis. Because of these activities, HO-1 has received much attention as a therapeutic target for a range of pathological processes.

Long Name:
Heme Oxygenase 1
Entrez Gene IDs:
3162 (Human); 24451 (Mouse); 15368 (Rat)
Alternate Names:
bK286B10; EC; heat shock protein, 32-kD; heme oxygenase (decycling) 1; HMOX1; HO; HO1; HO-1; HO-1heme oxygenase 1; HSP32

Citations for Human Total HO-1/HMOX1 DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Anti-Inflammatory Effect of Sparstolonin B through Inhibiting Expression of NF-kappaB and STAT-1
    Authors: N Kim, C Kim, SH Ryu, GO Kim, JS Bae
    International Journal of Molecular Sciences, 2022;23(18):.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Arginase 1 is a marker of protection against illness in contacts of leprosy patients
    Authors: RB da Silva P, MA Mendes, VC Soares, J França-Cos, AM Sales, NC Duppré, V de Matos B, TP da Silva, PT Bozza, MT Bozza, EN Sarno, MO Moraes, GM Sperandio, RO Pinheiro
    Scientific Reports, 2022;12(1):7850.
    Species: Human
    Sample Types: Serum
  3. Hydroxyurea treatment is associated with reduced degree of oxidative perturbation in children and adolescents with�sickle cell anemia
    Authors: CL Vinhaes, RS Teixeira, JAS Monteiro-J, R Tibúrcio, JM Cubillos-A, MB Arriaga, AG Sabarin, AJ de Souza, JJ Silva, IM Lyra, AM Ladeia, BB Andrade
    Sci Rep, 2020;10(1):18982.
    Species: Human
    Sample Types: Plasma
  4. Heightened Plasma Levels of Transforming Growth Factor Beta (TGF-&beta) and Increased Degree of Systemic Biochemical Perturbation Characterizes Hepatic Steatosis in Overweight Pediatric Patients: A Cross-Sectional Study
    Authors: JR Barretto, N Boa-Sorte, CL Vinhaes, H Malta-Sant, J Rebouças-S, CF Ramos, MAS Torres-Nas, VM Borges, BB Andrade
    Nutrients, 2020;12(6):.
    Species: Human
    Sample Types: Plasma
  5. Mangiferin Alleviates Ovalbumin-Induced Allergic Rhinitis via Nrf2/HO-1/NF-kappaB Signaling Pathways
    Authors: CH Piao, YJ Fan, TV Nguyen, CH Song, OH Chai
    Int J Mol Sci, 2020;21(10):.
    Species: Human
    Sample Types: Nasal Lavage Fluid
  6. Association of Omnivorous and Vegetarian Diets With Antioxidant Defense Mechanisms in Men
    Authors: N Cinegaglia, J Acosta-Nav, C Rainho, L Antoniazzi, S Mattioli, C Pimentel, RD Santos, V Sandrim
    J Am Heart Assoc, 2020;9(12):e015576.
    Species: Human
    Sample Types: Plasma
  7. Baicalin Exerts Anti-Airway Inflammation and Anti-Remodelling Effects in Severe Stage Rat Model of Chronic Obstructive Pulmonary Disease
    Authors: G Wang, N Mohammadtu, Y Lv, H Zhang, J Sun, J Dong
    Evid Based Complement Alternat Med, 2018;2018(0):7591348.
    Species: Rat
    Sample Types: Serum
    Authors: R Budiarti, Kuntaman, Nasronudin, Suryokusum, SQ Khairunisa
    Afr J Infect Dis, 2018;12(1):1-6.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Comparing the effects of mitochondrial targeted and localized antioxidants with cellular antioxidants in human skin cells exposed to UVA and hydrogen peroxide.
    Authors: Oyewole, Anne O, Wilmot, Marie-Cl, Fowler, Mark, Birch-Machin, Mark A
    FASEB J, 2014;28(1):485-94.
    Species: Human
    Sample Types: Whole Cells
  10. Determination of an angiotensin II-regulated proteome in primary human kidney cells by stable isotope labeling of amino acids in cell culture (SILAC).
    Authors: Konvalinka, Ana, Zhou, Joyce, Dimitromanolakis, Apostolo, Drabovich, Andrei P, Fang, Fei, Gurley, Susan, Coffman, Thomas, John, Rohan, Zhang, Shao-Lin, Diamandis, Elefther, Scholey, James W
    J Biol Chem, 2013;288(34):24834-47.
    Species: Human
    Sample Types: Cell Lysates
  11. Increased levels of plasma haemoxygenase-1 in prostate cancer.
    Authors: Blann AD, Balakrishnan B, Ryan P
    Prostate Cancer Prostatic Dis., 2011;14(2):114-7.
    Species: Human
    Sample Types: Plasma


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