Human Total MMP-13 DuoSet ELISA

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Human Total MMP-13 ELISA Standard Curve
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Product Details
Citations (10)
Supplemental Products
Reviews (1)

Human Total MMP-13 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Total MMP-13. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human Total MMP-13 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-13

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:
Matrix Metalloproteinase 13
Entrez Gene IDs:
4322 (Human)
Alternate Names:
CLG 3; CLG3EC 3.4.24; collagenase 3; EC 3.4.24.-; EC; EC; EC; EC; EC; MANDP1; matrix metallopeptidase 13 (collagenase 3); matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP13; MMP-13

Citations for Human Total MMP-13 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Exuberant fibroblast activity compromises lung function via ADAMTS4
    Authors: DF Boyd, EK Allen, AG Randolph, XJ Guo, Y Weng, CJ Sanders, R Bajrachary, NK Lee, CS Guy, P Vogel, W Guan, Y Li, X Liu, T Novak, MM Newhams, TP Fabrizio, N Wohlgemuth, PM Mourani, PALISI Ped, TN Wight, S Schultz-Ch, SA Cormier, K Shaw-Salib, A Pekosz, RE Rothman, KF Chen, Z Yang, RJ Webby, N Zhong, JC Crawford, PG Thomas
    Nature, 2020;0(0):.
    Species: Mouse
    Sample Types: BALF
  2. Thickness-wise growth technique for human articular chondrocytes to fabricate three-dimensional cartilage grafts
    Authors: T Kikuchi, T Shimizu
    Regen Ther, 2020;14(0):119-127.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Widespread regulation of gene expression by glucocorticoids in chondrocytes from patients with osteoarthritis as determined by RNA-Seq
    Authors: A Pemmari, T Leppänen, M Hämäläinen, T Moilanen, K Vuolteenah, E Moilanen
    Arthritis Res Ther, 2020;22(1):271.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Theaflavins prevent cartilage degeneration via AKT/FOXO3 signaling in�vitro
    Authors: J Li, J Zheng
    Mol Med Rep, 2019;19(2):821-830.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Clinical predictors of liver fibrosis in patients with chronic hepatitis B virus infection from children to adults
    Authors: JF Wu, SH Song, CS Lee, HL Chen, YH Ni, HY Hsu, TC Wu, MH Chang
    J. Infect. Dis., 2018;0(0):.
    Species: Human
    Sample Types: Serum
  6. PKR activation causes inflammation and MMP-13 secretion in human degenerated articular chondrocytes
    Authors: CH Ma, CH Wu, IM Jou, YK Tu, CH Hung, PL Hsieh, KL Tsai
    Redox Biol, 2017;14(0):72-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Saikosaponin A inhibits IL-1?-induced inflammatory mediators in human osteoarthritis chondrocytes by activating LXR?
    Authors: H Gao, Y Song, D Li, W Feng, J Liu
    Oncotarget, 2017;8(51):88941-88950.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Double-stranded RNA released from damaged articular chondrocytes promotes cartilage degeneration via Toll-like receptor 3-interleukin-33 pathway
    Authors: C Li, K Chen, H Kang, Y Yan, K Liu, C Guo, J Qi, K Yang, F Wang, L Guo, C He, L Deng
    Cell Death Dis, 2017;8(11):e3165.
    Species: Human
    Sample Types: Tissue Homogenates
  9. Dimethyl fumarate protection against collagen II degradation.
    Authors: Li Y, Tang J, Hu Y
    Biochem Biophys Res Commun, 2014;454(2):257-61.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro.
    Authors: Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R
    PLoS ONE, 2014;9(5):e96925.
    Species: Human
    Sample Types: Cell Culture Supernates


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Human Total MMP-13 DuoSet ELISA
By Jonatan Dereke on 08/06/2019
Sample Tested: EDTA Plasma

We ran human EDTA-plasma samples at a 1:5 dilution for this assay.