Human Total MMP-14/MT1-MMP DuoSet ELISA

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Human Total MMP-14 / MT1-MMP ELISA Standard Curve
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Product Details
Citations (5)
Supplemental Products
Reviews (3)

Human Total MMP-14/MT1-MMP DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human Total MMP-14 / MT1-MMP ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-14/MT1-MMP

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:
Matrix Metalloproteinase 14/Membrane Type 1 MMP
Entrez Gene IDs:
4323 (Human)
Alternate Names:
EC 3.4.24; EC; matrix metallopeptidase 14 (membrane-inserted); matrix metalloproteinase 14 (membrane-inserted); matrix metalloproteinase-14; membrane type 1 metalloprotease; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP14; MMP-14; MMP-X1; MT1MMP; MT1-MMP; MT1-MMPMTMMP1; MT-MMP 1; MT-MMP1

Citations for Human Total MMP-14/MT1-MMP DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Concerted actions by MMPs, ADAMTS and serine proteases during remodeling of the cartilage callus into bone during osseointegration of hip implants
    Authors: J Cassuto, A Folestad, J Göthlin, H Malchau, J Kärrholm
    Bone Rep, 2020;13(0):100715.
    Species: Human
    Sample Types: Plasma
  2. Inflammatory Bowel Disease Types Differ in Markers of Inflammation, Gut Barrier and in Specific Anti-Bacterial Response
    Authors: S Coufal, N Galanova, L Bajer, Z Gajdarova, D Schierova, Z Jiraskova, K Kostovciko, Z Jackova, Z Stehlikova, P Drastich, H Tlaskalova, M Kverka
    Cells, 2019;8(7):.
    Species: Human
    Sample Types: Serum
  3. Processed eggshell membrane powder regulates cellular functions and increase MMP-activity important in early wound healing processes
    Authors: TT Vuong, SB Rønning, TAE Ahmed, K Brathagen, V Høst, MT Hincke, HP Suso, ME Pedersen
    PLoS ONE, 2018;13(8):e0201975.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Functionally distinct disease-associated fibroblast subsets in rheumatoid arthritis
    Authors: F Mizoguchi, K Slowikowsk, K Wei, JL Marshall, DA Rao, SK Chang, HN Nguyen, EH Noss, JD Turner, BE Earp, PE Blazar, J Wright, BP Simmons, LT Donlin, GD Kalliolias, SM Goodman, VP Bykerk, LB Ivashkiv, JA Lederer, N Hacohen, PA Nigrovic, A Filer, CD Buckley, S Raychaudhu, MB Brenner
    Nat Commun, 2018;9(1):789.
    Species: Human
    Sample Types: Cell Lysates
  5. MMP-14 overexpression correlates with the neurodegenerative process in familial amyloidotic polyneuropathy
    Authors: D Martins, J Moreira, NP Gonçalves, MJ Saraiva
    Dis Model Mech, 2017;10(10):1253-1260.
    Species: Human
    Sample Types: Plasma


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Reviews for Human Total MMP-14/MT1-MMP DuoSet ELISA

Average Rating: 3.7 (Based on 3 Reviews)

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Human Total MMP-14/MT1-MMP DuoSet ELISA
By Anonymous on 07/06/2021
Sample Tested: Serum and Plasma

we used this kit to quantify human MMP14 in serum and plasma samples. Most of the samples are undetectable but the standard works ver well.

Human Total MMP-14/MT1-MMP DuoSet ELISA
By Jonatan Dereke on 04/30/2020
Sample Tested: EDTA Plasma

We ran human undiluted EDTA-plasma in this assay. Even though the analysis did perform good. The majority of samples had levels below detection range.

Human Total MMP-14/MT1-MMP DuoSet ELISA
By Anonymous on 11/07/2018
Sample Tested: Breast cancer cells