Human Total MMP-14/MT1-MMP DuoSet ELISA

R&D Systems | Catalog # DY918-05

R&D Systems
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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

0.625-20 ng/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human Total MMP-14/MT1-MMP DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all MMP-14/MT1-MMP ELISA Kits »

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human Total MMP-14/MT1-MMP DuoSet ELISA

Human Total MMP-14 / MT1-MMP ELISA Standard Curve

Human Total MMP-14 / MT1-MMP ELISA Standard Curve

Kit Contents for Human Total MMP-14/MT1-MMP DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)

Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)

Microplates: (Catalog # DY990), or equivalent

Plate Sealers: (Catalog # DY992), or equivalent

*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-14/MT1-MMP

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name

Matrix Metalloproteinase 14/Membrane Type 1 MMP

Alternate Names

MMP-X1, MMP14, MT-MMP1, MT1-MMP

Entrez Gene IDs

4323 (Human)

Gene Symbol

MMP14

Additional MMP-14/MT1-MMP Products

Product Documents for Human Total MMP-14/MT1-MMP DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Total MMP-14/MT1-MMP DuoSet ELISA

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human Total MMP-14/MT1-MMP DuoSet ELISA

Customer Reviews for Human Total MMP-14/MT1-MMP DuoSet ELISA (3)

3.7 out of 5
3 Customer Ratings
5 Stars
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3 Stars
67%
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  • Human Total MMP-14/MT1-MMP DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 07/06/2021
    we used this kit to quantify human MMP14 in serum and plasma samples. Most of the samples are undetectable but the standard works ver well.
    Human Total MMP-14/MT1-MMP DuoSet ELISA DY918-05
  • Human Total MMP-14/MT1-MMP DuoSet ELISA
    Name: Jonatan Dereke
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 04/30/2020
    We ran human undiluted EDTA-plasma in this assay. Even though the analysis did perform good. The majority of samples had levels below detection range.
    Human Total MMP-14/MT1-MMP DuoSet ELISA DY918-05
  • Human Total MMP-14/MT1-MMP DuoSet ELISA
    Name: Anonymous
    Sample Tested: Breast cancer cells
    Verified Customer | Posted 11/07/2018

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