Human Total TrkB DuoSet IC ELISA, 2 Plate

  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4 hours 40 mins (after plate preparation)
  • Sample Type & Volume Required
    Cell lysates (100 µL)
  • Range
    93.80 - 6,000 pg/mL
  • Sufficient Materials
    Kits available for two, five, or fifteen 96-well plates*
  • Specificity
    Please see the product datasheet
  • * Following cell simulation.
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure in cell lysates. An immobilized capture antibody specific for binds both phosphorylated and unphosphorylated . After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.


Product Features
  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot
Kit Content
  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP
Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*


Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Background: TrkB
Members of the Trk family (TrkA, B, and C) of receptor tyrosine kinases act as receptors for the neurotrophin family: BDNF, NGF, NT-3, and NT-4. Trks exhibit selectivity for specific neurotrophins. TrkA, TrkB and TrkC preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively. All Trk family proteins share a common structural organization consisting of an extracellular region with two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains, and an intracellular region that contains the tyrosine kinase domain. They are best known for their regulation of neuronal survival and axon outgrowth.
  • Entrez Gene IDs:
    4915 (Human); 18212 (Mouse); 25054 (Rat);
  • Long Name:
    Neurotrophic Tyrosine Kinase Receptor B
  • Aliases:
    NTRK2; BDNF/NT-3 growth factors receptor; EC 2.7.10; EC 2.7.10.1; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; Trk-B; trk-B; TrkB tyrosine kinase; TRKBBDNF-tropomyosine receptor kinase B; tyrosine kinase receptor B
Supplemental ELISA Products
Description Application Cat# Citations Images  

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)

DY999 35
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Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)

DY995 12
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Stop Solution 2N Sulfuric Acid (15 x 6 mL)

DY994 7
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ELISA Plate Sealers, 100 Pack

DY992
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Quantikine Wash Buffer 1

WA126 5
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Clear Polystyrene Microplates, 25 Pack

DY990 2
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Reagent Additive 1 (21 mL)

DY005
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ELISA Plate-coating Buffer (250 mL of PBS)

DY006 1
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Glo Substrate Reagent Pack (Reagent A & B)

DY993 2
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Sample Diluent Concentrate 2 (2X)

DYC002 1
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Sample Diluent Concentrate 1 (5X, 5 x 21 mL)

DYC001
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Reagent Diluent Concentrate 3 (5X, 10 x 21 mL)

DY004
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Black Polystyrene Microplates, 25 Pack

DY991 1
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