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Human Total TrkB DuoSet IC ELISA, 2 Plate

  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4 hours 40 mins (after plate preparation)
  • Sample Type & Volumne Required
    Cell lysates (100 µL)
  • Range
    93.80 - 6,000 pg/mL
  • Sufficient Materials
    Kits available for two, five, or fifteen 96-well plates*
  • Specificity
    Please see the product datasheet
  • * Following cell simulation.
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot
Kit Content
  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP
Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*


Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Background TrkB
  • Molecule Information:
    TrkB
  • Long Name:
    Neurotrophic Tyrosine Kinase Receptor B
  • Aliases:
    NTRK2; BDNF/NT-3 growth factors receptor; EC 2.7.10; EC 2.7.10.1; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; trk-B; Trk-B; TrkB tyrosine kinase; TRKBBDNF-tropomyosine receptor kinase B; tyrosine kinase receptor B
  • Entrez Gene IDs:
    4915 (Human); 18212 (Mouse); 25054 (Rat);
  • Background:
    TrkB
Members of the Trk family (TrkA, B, and C) of receptor tyrosine kinases act as receptors for the neurotrophin family: BDNF, NGF, NT-3, and NT-4. Trks exhibit selectivity for specific neurotrophins. TrkA, TrkB and TrkC preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively. All Trk family proteins share a common structural organization consisting of an extracellular region with two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains, and an intracellular region that contains the tyrosine kinase domain. They are best known for their regulation of neuronal survival and axon outgrowth.

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