Human Transglutaminase 3/TGM3 Antibody

Catalog # Availability Size / Price Qty
Detection of Human and Mouse Transglutaminase 3/TGM3 by Western Blot.
2 Images
Product Details
Supplemental Products

Human Transglutaminase 3/TGM3 Antibody Summary

Species Reactivity
Detects human Transglutaminase 3/TGM3 in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 3/TGM3
Accession # NP_003236
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Transglutaminase 3/TGM3 antibody by Western Blot. View Larger

Detection of Human and Mouse Transglutaminase 3/TGM3 by Western Blot. Western blot shows lysates of B16-F1 mouse melanoma cell line, Bowes human melanoma cell line, and human skin tissue. PVDF Membrane was probed with 1 µg/mL of Human Transglutaminase 3/TGM3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4604) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, Recombinant Human Transglutaminase 3/TGM3 (Catalog # 4604-TG) (10 ng/lane) uncleaved and cleaved by recombinant human Cathepsin were included. Specific bands were detected for uncleaved Transglutaminase 3/TGM3 at approximately 77 kDa (as indicated) and Transglutaminase 3/TGM3 cleavage products at approximately 50 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Transglutaminase 3/TGM3 antibody in Human Esophagus by Immunohistochemistry (IHC-P). View Larger

Transglutaminase 3/TGM3 in Human Esophagus. Transglutaminase 3/TGM3 was detected in immersion fixed paraffin-embedded sections of human esophagus using Human Transglutaminase 3/TGM3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4604) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cells in the papillae layer in mucosa. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transglutaminase 3/TGM3

Transglutaminase 3 (TG3), also known as epidermal Transglutaminase (Tgase E), belongs to the family of Transglutaminase enzymes that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1-3). TG3 is involved in the formation of the cornified envelope in skin keratinocytes (4). It functions to cross-link structural proteins during epidermal terminal differentiation. TG3 has been implicated as the dominant autoantigen in dermatitis herpetiformis (5). TG3 activation requires proteolysis of the 77 kDa zymogen into two fragments of approximately 50 and 27 kDa to form the active enzyme (1).

  1. Kim, I.G. et al. (1993) J. Biol. Chem. 268:12682.
  2. Griffin, M. et al. (2002) Biochem. J. 368:377.
  3. Lorand, L. and R.M. Graham (2003) Nat. Rev. Mol. Cell Biol. 4:140.
  4. Eckert, R.L. et al. (2005) J. Invest. Dermatol. 124:481.
  5. Sardy, M. et al. (2002) J. Exp. Med. 195:747.
Entrez Gene IDs
7053 (Human); 21818 (Mouse)
Alternate Names
EC; MGC126250; protein-glutamine gamma-glutamyltransferase E; TG(E); TG3; TGase E; TGase-3; TgaseE; TGEMGC126249; TGM3; transglutaminase 3 (E polypeptide, protein-glutamine-gamma-glutamyltransferase); Transglutaminase 3; Transglutaminase E; Transglutaminase-3

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Transglutaminase 3/TGM3 Antibody

There are currently no reviews for this product. Be the first to review Human Transglutaminase 3/TGM3 Antibody and earn rewards!

Have you used Human Transglutaminase 3/TGM3 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review