Human Tryptase beta-2/TPSB2 Antibody

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Human Tryptase beta-2/TPSB2 Antibody Summary

Species Reactivity
Detects human Tryptase beta ‑2/TPSB2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) TPS1, rhTPSG1, recombinant mouse (rm) TPS5, rmMCPT1, rmMCPT6, rmMCPT7, or rmMCPT11 is observed.
Monoclonal Mouse IgG1 Clone # 349414
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Tryptase beta ‑2/TPSB2
Accession # AAD13876
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human Tryptase  beta ‑2/TPSB2 (Catalog # 3796-SE)
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Tryptase  beta ‑2/TPSB2 (Catalog # 3796-SE), see our available Western blot detection antibodies
Measured by its ability to neutralize Recombinant Human Tryptase  beta ‑2/TPSB2 (0.5 µg/mL, Catalog # 3796-SE) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 1.4 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase beta-2/TPSB2

Tryptases are trypsin-like serine proteases, and beta  tryptases appear to be the main isoenzymes expressed in mast cells (1). They are stored in secretory granules of mast cells, where they form active tetramers with heparin proteoglycan. Because of the unique arrangement of the active sites in the tetramer, which are facing a narrow central pore, beta  tryptases are resistant to macromolecule protease inhibitors (2). When mast cells are activated, beta  tryptases are released along with other proteins in secretory granules, participating in provoking inflammatory conditions (3). beta  tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic disorders.

  1. Caughey, G. H. 2004, in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1535.
  2. Sommerhoff, C.P. et al. (1999) Proc. Natl. Acad. Sci. USA. 96:10984.
  3. Hallgren, J. and G. Pejler (2006) FEBS J. 273:1871.  
Entrez Gene IDs
64499 (Human); 17229 (Mouse); 29268 (Rat)
Alternate Names
EC 3.4.21; EC; mast cell tryptase beta II; mast cell tryptase beta III; TPS2; TPS2tryptase beta-2; TPSB2; tryptase beta 2 (gene/pseudogene); tryptase beta 2; tryptase beta II; tryptase beta III; Tryptase beta2; Tryptase beta-2; Tryptase II; tryptase III; tryptase-2; tryptaseB; tryptaseC; Tryptase-C

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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