Thyroid stimulating hormone receptor (TSH R; also thyrotropin receptor) is an 85 kDa (unglycosylated) member of the G-protein coupled receptor 1 family. Human TSH R is synthesized as a 764 amino acid (aa) precursor that contains a 20 aa signal sequence, a 393 aa extracellular domain (ECD), a 269 aa membrane spanning domain consisting of seven transmembrane segments, and an 82 aa cytoplasmic domain. The ECD contains six leucine-rich repeats (LRR) and six potential sites of N-linked glycosylation. There are two isoforms, long and short, produced by a splicing variant corresponding to aa 253-764 in the long isoform that is missing in the short isoform. Human TSH R is 86-87% aa identical to mouse and rat TSH R. TSH R is the receptor for thyroid stimulating hormone, which plays a central role in controlling thyroid cell metabolism.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Accession # P16473
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human TSHR Antibody
Human TSH R ELISA Standard Curve.
Recombinant human TSH R protein was serially diluted 2-fold and captured by Mouse Anti-Human TSH R Monoclonal Antibody (Catalog # MAB65341) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human TSH R Monoclonal Antibody (Catalog # MAB6534) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Applications for Human TSHR Antibody
ELISA
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human TSH R Monoclonal Antibody (Catalog # MAB6534).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Reviewed Applications
Read 1 review rated 5 using MAB65341 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TSHR
Long Name
Alternate Names
Gene Symbol
UniProt
Additional TSHR Products
Product Documents for Human TSHR Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human TSHR Antibody
For research use only
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Application: ELISASample Tested: SerumSpecies: HumanVerified Customer | Posted 04/03/2018
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Protocols
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